‘紫娟’茶花色苷的分离鉴定  

Separation and Identification of Anthocyanins from ‘Zijuan’ Tea Leaves

作　　者：李燕丽[1,2] 罗琼仙[3] 杨雪梅[1] 占琪 李家华[1] LI Yanli;LUO Qiongxian;YANG Xuemei;ZHAN Qi;LI Jiahua(College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, China;Tea Industry Development Center of Meitan,Meitan 564100, China;Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, China)

机构地区：[1]云南农业大学龙润普洱茶学院,云南昆明650201 [2]湄潭县茶产业发展中心,贵州湄潭564100 [3]云南省农业科学院茶叶研究所,云南勐海666201

出　　处：《食品科学》2017年第12期125-130,共6页Food Science

基　　金：国家自然科学基金地区科学基金项目(31360196)

摘　　要：依次以MCI gel CPH 20P(75~150μm)树脂和Sephadex^(TM) LH-20葡聚糖凝胶为层析柱填料,对‘紫娟’茶花色苷进行分离纯化,采用5%乙酸-甲醇溶液和5%乙酸溶液对花色苷提取液梯度洗脱,得到6种花色苷组分。采用薄层层析、高效液相色谱及高效液相色谱-电喷雾-串联质谱对‘紫娟’茶花色苷组成成分进行研究。结果表明:从‘紫娟’茶鲜叶中分离出的花色苷为飞燕草素-3-O-半乳糖苷、矢车菊素-3-O-半乳糖苷、飞燕草素-3-O-(6-(Z)对香豆酸)吡喃半乳糖苷、矢车菊素-3-O-(6-(Z)对香豆酸)吡喃半乳糖苷、飞燕草素-3-O-(6-(E)对香豆酸)吡喃半乳糖苷、矢车菊素-3-O-(6-(E)对香豆酸)吡喃半乳糖苷。The anthocyanins from leaves of the tea cultivar‘Zijuan’were separated and purified by sequential columnchromatography using MCI gel CHP20P resin(75–150μm)and SephadexTM LH-20,through gradient elution with5%acidmethanoland5%acid-water.A total of6anthocyanins were separated from the purified extract,which were analyzed byhigh performance liquid chromatography(HPLC),thin layer chromatography(TLC)and HPLC-electrospray ionizationtandemmass spectrometry(HPLC-ESI-MS/MS).The results showed that the anthocyanins were identified as delphinidin3-O-β-D-galactoside,cyanidin3-O-β-D-galactoside,delphinidin3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside,cyanidin3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside,delphinidin3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside and cyanidin3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside.

关 键 词：'紫娟’茶 花色苷 分离纯化 高效液相色谱-电喷雾-串联质谱法 结构鉴定 

分 类 号：TS272.2[农业科学—茶叶生产加工] Q946.8[轻工技术与工程—农产品加工及贮藏工程]