紫山药多糖对D-半乳糖衰老模型大鼠肝、脑的影响  

Effect of Purple Yam Polysaccharide on the Liver and Brain of D-Galactose Induced Aging Rats

作　　者：张丽梅[1,2] 程永强[1] 宋曙辉[3] ZHANG Limei;CHENG Yongqiang;SONG Shuhui(Beijing Key Laboratory for Functional Foods from Plant Resources, College of Food Science and Nutritional Engineering,China Agricultural University, Beijing 100083, China;New Era Health Industry (Group) Co. Ltd., Beijing 102206, China;National Engineering Research Center for Vegetables, Beijing 100080, China)

机构地区：[1]中国农业大学食品科学与营养工程学院植物源功能食品北京市重点实验室,北京100083 [2]新时代健康产业(集团)有限公司,北京102206 [3]国家蔬菜工程技术研究中心,北京100080

出　　处：《食品科学》2017年第13期196-200,共5页Food Science

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20140111;KJCX20150201)

摘　　要：目的:探讨紫山药多糖对D-半乳糖所致衰老模型大鼠的抗衰老作用及其作用机理。方法:以D-半乳糖建立大鼠衰老模型,灌胃紫山药多糖(20、100、500 mg/(kg·d))45 d后,检测大鼠肝、脑组织中总抗氧化能力(total antioxidant capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,检测谷胱甘肽(glutathione,GSH)及过氧化终产物丙二醛(malondialdehyde,MDA)含量,应用免疫印迹实验评价肝、脑中p53、p21蛋白的表达。结果:100 mg/(kg·d)紫山药多糖可显著提高D-半乳糖衰老模型大鼠肝、脑中T-AOC、GSH-Px活力、GSH含量,降低过氧化产物MDA含量,抑制衰老基因p53、p21的蛋白表达。结论:紫山药多糖具有显著的抗大鼠肝、脑衰老损伤的作用,作用机理可能与p53/p21信号通路有关。Objective:To study the anti-aging effect and mechanism of purple yam polysaccharide(PYP)on D-galactoseinduced aging model rats.Methods:The aging rat model was established by D-galactose injection for45days andadministered simultaneously with PYP at20,100and500mg/(kg·d),and total antioxidant capacity(T-AOC),glutathioneperoxidase(GSH-Px)activities and glutathione(GSH)and malondialdehyde(MDA)contents in brain and liver tissues weredetected after the administration.The expression of p53and p21protein was evaluated using Western blot.Results:PYP at100mg/(kg·d)could improve T-AOC,GSH-Px and GSH contents in liver and brain tissues of D-galactose induced agingrats,decrease the production of MDA,and down-regulate the expression of p53and p21protein.Conclusion:Purple yampolysaccharide has a significant effect on defending against liver and brain aging in rats via a mechanism related to p53/p21signaling pathways.

关 键 词：紫山药多糖 衰老 P53蛋白 P21蛋白 

分 类 号：TS201.4[轻工技术与工程—食品科学]