番茄红素对糖尿病小鼠骨髓内皮祖细胞功能影响及机制  

Effect and Mechanism of Lycopene on Functions of Endothelial Progenitor Cells in STZ-Induced Diabetic Mice

作　　者：曾瑶池 黄淑芬[2] 徐华[3] 穆桂萍[4] 周熙临 彭立生[5] ZENG Yaochi;HUANG Shufen;XU Hua;MU Guiping;ZHOU Xilin;PENG Lisheng(Department of Clinical Nutrition, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China;Department of Health Education, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China;Department of Pathology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China;Department of Central Laboratory, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China;Department of Science and Education, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China)

机构地区：[1]深圳市中医院营养科,广东深圳518033 [2]深圳市中医院健康教育科,广东深圳518033 [3]深圳市中医院病理科,广东深圳518033 [4]深圳市中医院中心实验室,广东深圳518033 [5]深圳市中医院科教科,广东深圳518033

出　　处：《食品科学》2017年第13期201-206,共6页Food Science

基　　金：广东省科技计划项目(2014A020212525)

摘　　要：以链脲霉素(streptozotocin,STZ)诱导C57BL/6小鼠作为糖尿病动物模型,探究番茄红素(lycopene,Lyc)对STZ诱导的糖尿病小鼠骨髓内皮祖细胞(endothelial progenitor cells,EPCs)的保护作用,探讨Lyc防治糖尿病血管并发症的潜力。结果表明:糖尿病小鼠外周血EPCs数量减少,骨髓EPCs氧化应激水平增高,血管内皮生长因子(vascular endothelial growth factor,VEGF)表达降低,EPCs衰老严重并伴功能受损。Lyc灌胃处理糖尿病小鼠后,其外周血EPCs数量增加,骨髓EPCs氧化应激得到抑制,VEGF表达增加,EPCs衰老和功能受损程度明显减轻。由此可知,Lyc作为一种食物来源的植物化合物,具有防治糖尿病血管并发症的潜力。C57BL/6mice challenged with streptozotocin(STZ)were used as an animal model of diabetes mellitus(DM)to study the protective effect of lycopene(Lyc)on functions of endothelial progenitor cells(EPCs)to evaluate its potentialin the prevention and treatment of DM vascular complications.The results showed that a significant difference in thenumber of EPCs derived from peripheral blood was observed between diabetic and normal mice.The migration ability,tubeformation ability and vascular endothelial growth factor(VEGF)protein expression level of bone marrow-derived EPCsin diabetic mice were obviously lower than those in normal mice.STZ accelerated oxidative stress and the onset of EPCssenescence.Based on our results,long-term administration with30mg/kg Lyc significantly relieved oxidative stress and thesenescence of EPCs,and increased the VEGF protein expression level,migration ability and tube formation ability of EPCswhen compared with the model group.Lycopene,as a food phytochemical,has the potential to be used in the prevention andtreatment of DM vascular complications.

关 键 词：番茄红素 内皮祖细胞 链脲霉素 糖尿病 氧化损伤 

分 类 号：R587.1[医药卫生—内分泌]