2-甲氧基雌二醇联用抗坏血酸诱导慢性粒细胞白血病细胞凋亡及细胞周期变化研究  

Study on apoptosis and cell cycle changes of CML cells induced by 2-Methoxy estradiol combined with ascorbic acid

作　　者：张苏伟[1] 曾嫦[1] 林祥伟[1] ZHANG Suwei;ZENG Chang;LIN Xiangwei(Department of Laboratory, Shantou Central Hospital, Shantou 515031, China)

机构地区：[1]广东省汕头市中心医院检验科,广东汕头515031

出　　处：《中国医药科学》2017年第11期18-21,共4页China Medicine And Pharmacy

基　　金：广东省汕头市科技计划项目(汕府科[2015]123-6)

摘　　要：目的研究2-ME联合抗坏血酸在诱导CML细胞凋亡中的作用以及细胞周期的变化机制。方法收集2016年1~12月期间汕头市中心医院血液科已确诊的bcr/abl(+)慢性粒细胞白血病患者57例骨髓,应用流式细胞术分选出CML细胞并培养传代并随机分为四组。使用抗肿瘤化合物2-甲氧基雌二醇(2-ME)与抗坏血酸(AA)组合来处理慢性粒细胞白血病(CML)细胞,根据药物浓度分为:(1)未加药组;(2)2-ME组(2μmol/L);(3)AA组(62.5μmol/L);(4)2-ME联用AA组(2-ME:2μmol/L+AA:62.5μmol/L)四组。应用流式细胞术分析药物处理后的CML细胞凋亡情况和细胞周期的变化情况。结果用(±s)表示,两组间比较采用t检验,多组间比较用F检验。结果 2μmol/L 2-ME+62.5μmol/LAA组可诱导CML细胞凋亡。2-ME+AA以剂量依赖性方式诱导凋亡,在浓度2~8μmol/L时凋亡呈时间-剂量依赖。2-ME+AA可诱导处于G0/G1期的细胞明显增多,处于S期的细胞减少。结论 2-ME联合抗坏血酸可诱导白血病细胞以时间-剂量依赖式凋亡,且与细胞周期抑制有关。Objective To study the role of2-ME combined with ascorbic acid in the induction of CML cell apoptosis and the mechanism of cell cycle.Methods57Bone marrow of patients who diagnosed bcr/abl(+)chronic myeloid leukemia in Department of Hematology,Shantou Central Hospital between January2016and December2016,was collected.And CML cells were sorted out by flow cytometry.CML cells were randomly divided into four groups.2-methoxy estradiol(2-ME)combined with ascorbic acid(AA)was used to treat chronic myeloid leukemia(CML)cells.According to the drug concentration is divided into four groups:(1)control;(2)2-MEgroup(2μmol/L);(3)AA group(62.5μmol/L);(4)2-ME combined with AA group(2-ME:2μmol/L+AA:62.5μmol/L).Flow cytometry was used to analyze the apoptosis and cell cycle of CML cells.Results CML cell apoptosis was induced by2μmol/L2-ME+62.5μmol/L AA group.2-ME+AA induced apoptosis in a dose-dependent manner,and apoptosis was time-dose-dependent at the concentration(2-8μmol/L).2-ME+AA can induce cells in G0/G1phase increased significantly,in the S phase of the cells decreased.Conclusion2-ME combined with ascorbic acid can induce leukemia cells in time-dose-dependent apoptosis,and is related to cell cycle inhibition.

关 键 词：白血病细胞 2-ME 抗坏血酸 流式细胞术 凋亡 周期变化 

分 类 号：R733.7[医药卫生—肿瘤]