EGCG纳米硒的稳定性及其对硒吸收利用的影响  

The Evaluation of the Stability of EGCG-Selenium Nanoparticles and Its Effect on Selenium Absorption and Utilization

作　　者：王乐[1] 李欢[1] 李嘉豪[1] 陈暄[1] 黎星辉[1] 孙康[1] WANG Le;LI Huan;LI Jiahao;CHEN Xuan;LI Xinghui;SUN Kang(Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China)

机构地区：[1]南京农业大学茶叶科学研究所,江苏南京210095

出　　处：《茶叶科学》2017年第4期373-382,共10页Journal of Tea Science

基　　金：江苏省自然科学基金(BK20160735);江苏省博士后科学基金(1601078C);现代农业产业技术体系建设专项资金(CARS-23);江苏高校优势学科建设工程资助项目

摘　　要：使用维生素C(Vitamin C,Vc)作为氧化还原剂和(-)-表没食子儿茶素没食子酸酯(EGCG)作为分散剂,成功制备了纳米硒(EGCG-SeNPs)。使用透射电子显微镜(TEM)和激光粒度分析仪电动电势仪(Zetasizer)测定的EGCG-SeNPs是平均直径为(35±0.12)nm、Zeta电位为-0.05 mV的球形颗粒。EGCG-SeNPs在pH1.0的强酸或70℃高温环境下发生聚集,其颗粒大小增加大约10倍。而且,EGCG-SeNPs中的EGCG作为分散剂具有良好的稳定性。通过分别灌胃25、50、100μg·kg^(-1)(以含Se量计算)的亚硒酸钠和EGCG-SeNPs发现,肝脏和肾脏中的硒含量在50、100μg·kg^(-1)的硒处理后均显著增加;在血清、肝脏和肾脏中,所有处理组的谷胱甘肽过氧化物酶(GPx)活性均显著增加,且具有剂量依赖效应。然而,在相同剂量的亚硒酸钠和EGCG-SeNPs处理组中,硒含量和GPx活性之间没有显著差异。因此可以得出,Vc作为氧化还原剂制备的EGCG-SeNPs可能具有和亚硒酸钠相似的生物利用度。(-)-epigallocatechin-3-gallate(EGCG)dispersed Selenium nanoparticles(EGCG-SeNPs)were prepared using vitamin C(Vc)and EGCG as the redox agent and dispersant.Characteristics of EGCG-SeNPs,which were determined using transmission electron microscope(TEM)and zetasizer,were spherical in shape with a mean diameter of(35±0.12)nm and-0.05mV zeta potential.The particles were aggregated in strong acid and high temperature conditions(pH1.0and70℃),with the particle size increased by about10times.And,EGCG in EGCG-SeNPs had good stability as a dispersant.With the administration of25,50and100μg·kg-1(Calculated with selenium content),selenium content in liver and kidney of50and100μg·kg-1sodium selenite and EGCG-SeNPs treated mice were significantly increased.Glutathione peroxidase(GPx)activity in serum,liver and kidney of all the treatment groups were significantly increased in a dose-dependent manner.However,there was no significant difference between sodium selenite and EGCG-SeNPs at the same dose on selenium content and GPx activity.Hence,it can be concluded that EGCG-SeNPs synthesized using Vc as the redox agent might have the similar bioavailability to sodium selenite.

关 键 词：(-)-表没食子儿茶素-3-没食子酸酯 硒 纳米颗粒 生物利用度 谷胱甘肽过氧化物酶活性 

分 类 号：TS272[农业科学—茶叶生产加工] Q946.841[轻工技术与工程—农产品加工及贮藏工程]