枇杷叶科罗索酸抑制人低密度脂蛋白氧化修饰及保护血管内皮细胞氧化损伤作用  

Corosolic Acid from Folium Eriobotryae Inhibits LDL Oxidation and Protects HAECs against Oxidative Damage

作　　者：李锋[1,2] 李义嘉 李清仙 郭养浩[2] 石贤爱[2] LI Feng;LI Yijia;LI Qingxian;GUO Yanghao;SHI Xian-ai(College of Chemistry, Fuzhou University, Fuzhou 350116, China;College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China)

机构地区：[1]福州大学化学学院,福建福州350116 [2]福州大学生物科学与工程学院,福建福州350116

出　　处：《食品科学》2017年第15期215-220,共6页Food Science

基　　金：国家海洋公益性行业科研专项(201205022)

摘　　要：从枇杷叶中提取分离制备科罗索酸,采用体外Cu^(2+)诱导低密度脂蛋白(low density lipoprotein,LDL)氧化损伤的体外化学反应模型及2,2’-偶氮二(2-甲基丙基咪)二盐酸盐(2,2’-azobis-2-methyl-propanimidamide dihydrochloride,AAPH)诱导人主动脉内皮细胞(human aortic endothelial cells,HAECs)氧化损伤的细胞模型,考察枇杷叶科罗索酸对LDL氧化过程的抑制作用及对动脉血管内皮细胞氧化损伤的保护作用。体外实验结果表明,枇杷叶科罗索酸在体外10~100μmol/L剂量范围内能有效延长LDL氧化过程中的迟滞期,降低反应动力学曲线的曲线下面积以及降低脂质过氧化物的生成,表明科罗索酸在体外能有效抑制Cu^(2+)诱导的LDL氧化。细胞实验结果表明,在2~10μmol/L剂量范围内能有效降低AAPH所致HAECs的乳酸盐脱氢酶漏出量,维护细胞结构的完整性;提高受损细胞的超氧化物歧化酶、谷胱甘肽过氧化物酶活性,从而提升内皮细胞抵抗氧化应激的能力;降低细胞处于sub-G1/G0状态的比例,减少AAPH所致细胞的坏死或凋亡。表明枇杷叶科罗索酸能在细胞水平有效保护HAECs免受AAPH氧化应激损伤。In the present study,corosolic acid(CA)was extracted and separated from Folium Eriobotryae.A Cu2+-induced low density lipoprotein(LDL)oxidation model was employed to evaluate the inhibitory effect of CA on LDL oxidation in vitro,and a human aortic endothelial cell(HAEC)model of oxidative damage induced by(2,2'-azobis-2-methylpropanimidamide dihydrochloride,AAPH)was employed to evaluate the protective effect of CA on oxidative damage.In the dose range of10-100μmol/L,CA effectively extended the lag time of the Cu2+-induced LDL oxidation process,reduced the area under the oxidation kinetic curve(AUC)and inhibited the generation of lipid peroxide indicating that CA could effectively inhibit LDL oxidation induced by Cu2+.In the cellular experiment at the dose range of2-10μmol/L,CA effectively reduced LDH leakage induced by AAPH,maintained the integrity of cell structure,enhanced the activities of antioxidant enzymes such as superoxide dismutase(SOD)and glutathione peroxidase(GPx)and therefore oxidative stress resistance,reduced the proportion of sub-G1/G0cells and necrosis or apoptosis induced by AAPH.These results demonstrated that CA could elevate cellular antioxidant capacity,maintain the integrity of cellular structure,and ultimatelyp rotect HAECs against oxidative stress damage induced byA APH.

关 键 词：枇杷叶 科罗索酸 低密度脂蛋白 血管内皮细胞 脂质过氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]