路德维希肠杆菌EM1对二氯喹啉酸和Cd2+复合污染的修复  

Bioremediation of the combined pollution of quinclorac and Cd^(2+) by Enterobacter ludwigii EM1

作　　者：徐淑霞[1] 杜文涛[1] 王晓雅[1] 张继冉[1] 赵培[1] 吴坤[1] XU Shu-xia;DU Wen-tao;WANG Xiao-ya;ZHANG Ji-ran;ZHAO Pei;WU Kun(Key Laboratory of Enzyme Engineering, College of life sciences, Henan Agricultural University, Zhengzou 450002, China)

机构地区：[1]河南农业大学生命科学学院,农业部农业微生物酶工程重点实验室,河南郑州450002

出　　处：《中国环境科学》2017年第8期3159-3165,共7页China Environmental Science

基　　金：河南省重点科技攻关项目(152102110054);河南省高等学校重点科研项目(15A180005);河南省高校科技创新团队支持计划(15IRTSTHN014)

摘　　要：为解决稻田水体环境中农药与重金属的复合污染问题,利用扫描电镜、傅里叶红外光谱等分析技术,研究了路德维希肠杆菌(Enterobacter ludwigii)EM1对二氯喹啉酸-Cd^(2+)复合污染的修复机理.结果表明,EM1能在以二氯喹啉酸为单一碳源的条件下对Cd^(2+)进行吸附.在温度为35℃,p H为6.0的条件下培养7d,EM1对50mg/L的二氯喹啉酸和Cd^(2+)的降解及吸附率达到最大,分别为30%和60%.扫描电镜结果表明,复合修复过程中菌体形态发生变化,菌体表面分泌大量胞外聚合物.红外扫描分析显示,胁迫条件下菌体细胞壁结构并未发生变化,菌株降解二氯喹啉酸和吸附Cd^(2+)的过程主要与菌体表面的羟基、酰胺基、糖环中的C-O-C及脂肪族化合物有关.Basing on the study of degradation and adsorption characteristics of quinclorac and cadmium,bacteria EM1was used as the test strain.The interaction mechanism of quinclorac-Cd2+combined pollution with Enterobacter ludwigii EM1was investigated by using SEM and FTIR analytical techniques.The experimental results showed that the strains could adsorb Cd2+with quinclorac as the sole carbon source.After7d culture at a temperature of35℃,pH6.0,the degradation and adsorption of quinclorac and Cd2+in50mg/L reached the maximum,which were30%and60%respectively.The SEM results showed that in the process of composite repair the morphology of the cells was changed and a large number of extracellular polymeric substances were secreted in the cell surface.Infrared scanning analysis showed that the changes had not taken place in the cell walls under the stress condition.Hydroxy,aminoacyl,carbon-oxygencarbon bond in the sugar ring and the aliphatic compounds were confirmed to be the key functional groups for the strain to biodegrade quinclorac and adsorb Cd2+.

关 键 词：二氯喹啉酸 CD 路德维希肠杆菌EM1 吸附 复合污染 

分 类 号：X172[环境科学与工程—环境科学]