Expression pattern of BIM,BCL-6,and c-MYC in adult B-cell acute lymphoblastic leukemia  

作　　者：Chanli Zheng Lin Xu Yanjun Xie Dongmei He Yangqiu Li 

机构地区：[1]Institute of Hematology,Medical College,Jinan University,Guangzhou 510632,China [2]Department of Obstetrics and Gynecology,Panyu District Zhongcun Hospital,Guangzhou 511495,China [3]Key Laboratory for Regenerative Medicine of Ministry of Education,Jinan University,Guangzhou 510632,China

出　　处：《Oncology and Translational Medicine》2017年第4期151-155,共5页肿瘤学与转化医学（英文版）

基　　金：Supported by the gants of the Guangdong Province Key Foundation of Science and Technology Program(No.2009B0507000029);the Major Research Plan of the National Natural Science Foundation of China(cultivating project,No.91129720);the Guangdong Science&Technology Project(Nos.2012B050600023 and 2015A050502029);a Grant from the Overseas Chinese Affairs Office of the State Council Key Discipline Construction Fund(No.51205002)

摘　　要：Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia(B-ALL).Methods Relative m RNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference gene.Results Relative expression of BIM, BCL-6, and c-MYC m RNA in B-ALL patients was significantly lower than in healthy controls(P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL patients and those incomplete remission(CR)(P < 0.05). There were no statistically significant differences in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups(P > 0.05). Spearman's rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL patients.Conclusion Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia(B-ALL).Methods Relative m RNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference gene.Results Relative expression of BIM, BCL-6, and c-MYC m RNA in B-ALL patients was significantly lower than in healthy controls(P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL patients and those incomplete remission(CR)(P < 0.05). There were no statistically significant differences in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups(P > 0.05). Spearman's rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL patients.Conclusion Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.

关 键 词：BIM BCL-6 C-MYC B cell acute LYMPHOBLASTIC leukemia (ALL) quantitative REVERSETRANSCRIPTION polymerase chain reaction (qRT-PCR) 

分 类 号：R733.7[医药卫生—肿瘤]