Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens  

作　　者：Manjesh SAAKRE Thirthikar Meera BABURAO Abida Puthenpeedikal SALIM Rose Mary FFANCIES Valasala Poothecty ACHUTHAN George THOMAS Sajeevan Radha SIVARAJAN 

机构地区：[1]Centre for Plant Biotechnology and Molecular Biology/Department of Genetics and Plant Breeding/Department of Agronomy, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur 680656, Kerala, India

出　　处：《Rice science》2017年第5期291-298,共8页水稻科学（英文版）

基　　金：the Jawaharlal Nehru University(JNU) research fellowship sponsored by the Department of Biotechnology(DBT),Government of India

摘　　要：Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential.Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress.In the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,Pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and sequenced.Real-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1.This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage.These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential.Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress.In the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,Pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and sequenced.Real-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1.This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage.These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.

关 键 词：RICE drought tolerance PSEUDOMONAS FLUORESCENS differential display reverse transcription POLYMERASE chain reaction quantitative real-time PCR TRANSCRIPT derived fragment 

分 类 号：S511[农业科学—作物学]