超声波协同复合酶法提取桑黄多糖工艺优化  

Ultrasonic-assisted Enzymatic Method Extraction of Polysaccharide from Inonotus sanghuang

作　　者：程俊文[1] 贺亮[1] 魏海龙[1] 宋吉玲[2] 胡传久[1] 邹景泉[1] 李海波[1] 蒋云鹤[1] CHENG Jun-wen;HE Liang;WEI Hai-long;SONG Ji-ling;HU Chuan-jiu;ZOU Jing-quan;LI Hai-bo;JIANG Yun-he(Zhejiang Academy of Forestry, Key Laboratory of Biological and Chemical Utilization of Forest Resources, Hangzhou 310023, China;Hangzhou Academy of Agricultural Sciences of Zhejiang, Hangzhou 310024, China)

机构地区：[1]浙江省林业科学研究院浙江省森林资源生物与化学利用重点实验室,浙江杭州310023 [2]杭州市农业科学研究院,浙江杭州310024

出　　处：《浙江林业科技》2017年第4期33-38,共6页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省科技厅院所专项(2014F50018);国家自然科学基金项目(31501815);浙江省农业(食用菌)新品种选育项目(2016C02057-8);杭州市科委种子种苗专项(20150932H07)的部分研究内容

摘　　要：在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化桑黄Inonotus sanghuang子实体中多糖的超声波协同复合酶法提取工艺条件。在前期预实验的基础上,采用纤维素酶、果胶酶和蛋白酶按2:1:1组成复合酶对桑黄子实体进行酶解处理,添加量为2%,温度50℃,p H 6.5,在水溶液中酶解1 h。然后对工艺中的超声波功率、超声时间、液料比进行优化。响应面分析结果表明,桑黄子实体中多糖的最佳提取条件为:超声功率360.6 W,液料比32.5:1,超声时间32.7 min,多糖得率可得3.31%,与理论值3.46%基本吻合。Extraction technology of polysaccharide from Inonotus sanghuang was optimized by ultrasonic-assisted enzymatic method and response surface analysis.Based on the results of single factor experiment,enzymolysis of I.sanghuang sporcarp was carried out with2%of complex phosphoesterasum(ratio of cellulose,pectinase,protease of2:1:1)at50℃under pH of6.5for1hour.Then optimization of extraction technology was implemented on ultrasonic power,ultrasound time and ratio of liquid and material.The response surface analysis demonstrated that polusaccharide yield topped3.31%under condition as follows:ultrasonic power360.6W,liquid/material ratio32.5:1,ultrasound treatment time32.7minutes,similar to theoretical value of3.46%.

关 键 词：桑黄 多糖 超声波 复合酶 响应面 

分 类 号：TQ461[化学工程—制药化工]