食品接触类纸、木制品中杀菌剂的测定  

Detection of Fungicides in Food Contact Paper and Wood Products

作　　者：景俊谦 曹悦 王军[1] JING Junqian;CAO Yue;WANG Jun(College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2017年第20期256-261,共6页Food Science

基　　金：国家质量监督检验检疫总局项目(JDS201601)

摘　　要：为测定食品接触类纸、木制品中杀菌剂的含量,建立高效液相色谱-串联质谱同时分析5种常用杀菌剂(5-氯-2-甲基-4-异噻唑啉-3-酮、2-甲基-4-异噻唑啉-3-酮、1,2-苯并异噻唑啉-3-酮、2-溴-2-硝基-1,3-丙二醇(溴硝醇)、五氯苯酚)的方法。样品经过甲醇超声提取后,以甲醇和水为流动相,根据保留时间不同,采用电喷雾正、负离子分段离子化,多反应监测模式,外标法进行分析。结果表明:5种杀菌剂在1~100μg/L范围内线性良好,方法的检测限在2.7~47μg/kg之间,加标回收率为82.88%~106.31%,相对标准偏差为0.5%~8.1%。该方法灵敏、准确,适合食品接触类纸、木制品中杀菌剂含量的分析,可为监测该类产品对食品安全的影响提供一种有效的测定方法。A method was developed to simultaneously analyze the contents of five bactericides including2-methyl-4-isothiazoline-3-one(MI),5-chloro-2-methyl-3(2H)-isothiazolone(CMI),1,2-benzisothiazolin-3-one(BIT),pentachlorophenol(PCP)and bronopol,in food contact paper and wood products using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Samples were extracted ultrasonically with methanol.The analytes were separated on an Eclipse Plus column C18(4.6mm×100mm,3.5μm)with methanol-water as the mobile phase.The multiple reaction monitoring(MRM)mode was applied to qualitatively analyze the five bactericides with a positive or negative electrospray ionization source according to their retention times and characteristic structures.The external standard method was used for quantitative analysis.The results showed that the linear ranges of the five bactericides were from1to100μg/L,and the limit of detection(LOD)of the method(RSN=3)was in the range from2.7to47μg/kg.The mean recoveries for paper and wood samples at spiked concentration levels of8to500μg/kg ranged from82.88%to106.31%,and the precision relative standard deviations(n=5)were0.5%-8.1%.The method was applicable to analyze the contents of the five bactericides in food contact paper and wood products and therefore ensure food safety.

关 键 词：杀菌剂 食品接触类纸制品 食品接触类木制品 高效液相色谱-串联质谱联用法 

分 类 号：O657.63[理学—分析化学]