氮素水平对红花大金元烟叶游离氨基酸和蛋白质含量的影响  

Effect of Nitrogen Level on Free Amino Acid and Protein Contents of Honghuadajinyuan Tobacco Leaves

作　　者：黄茹[1] 吴玉萍[2] 夏振远[2] 王正银[3] 李银科[4] HUANG Ru;WU Yuping;XIA Zhenyuan;WANG Zhengyin;LI Yinke(Key Laboratory of Land-use in Plateau Mountainous Region, Yunnan University of Finance and Economics, Kunming 650221,China;Yunnan Academy of Tobacco Agricultural Science, Yuxi, Yunnan 650106, China;College of Resources and Environment,Southwest University, Chongqing 400716, China;College of Resources and Environment, Yuxi Normal University, Yuxi, Yunnan 631500, China)

机构地区：[1]云南财经大学,高原山地土地利用重点实验室,昆明650221 [2]云南省烟草农业科学研究院,云南玉溪650106 [3]西南大学资源环境学院,重庆400716 [4]玉溪师范学院资源环境学院,云南玉溪631500

出　　处：《中国烟草科学》2017年第5期50-55,共6页Chinese Tobacco Science

基　　金：中国烟草总公司重大专项“不同香型烟叶化学成分研究”(2012GC01)

摘　　要：为了解氮素水平对云南烟区红花大金元烟叶游离氨基酸和蛋白质含量的影响,通过田间试验设置4个施氮水平0、45、90和135 kg/hm^2,分析其对红花大金元烟叶游离氨基酸和蛋白质含量的影响,并通过感官质量对烟叶品质进行评价。结果表明,烟叶内组氨酸和天冬酰胺等含量随施氮水平的提高而显著增加,谷氨酰胺和精氨酸等含量以施氮90 kg/hm^2处理最高,施氮135 kg/hm^2时出现下降趋势,赖氨酸含量则是随施氮水平的增加而显著降低;游离氨基酸总量和蛋白质含量随施氮水平的提高显著增加,施氮135 kg/hm^2最高可达9.617 mg/g和10.56%;感官评吸中烟叶的香气质和评吸总分以施氮90kg/hm^2处理得分最高,不施氮处理最低。在本试验条件下,烟叶内游离氨基酸的含量变化随施氮水平的改变呈现出不一样的特点,但游离氨基酸总量和蛋白质含量随施氮水平的提高显著增加,施氮90 kg/hm^2处理烟叶感官质量最佳。Four nitrogen levels of N0,45,90and135kg/ha were setup to investigate the free amino acid and protein contents in Honghuadajinyuan(Hongda)tobacco at different nitrogen application levels.The results showed that histidine and asparagines content of tobacco increased significantly with the nitrogen application level.Glutamine and arginine contents in the90kg/ha N treatments were the highest,and decreased in the135kg/ha N treatment.Lysine content was decreased with the nitrogen levels increased.The total amount of free amino acids and protein content of tobacco increased significantly with the nitrogen application level,with up to9.617mg/g and10.56%in the135kg/ha N treatment.The quality of aroma and sensory quality reached the highest in90kg/ha N and the lowest in0N treatment.Under the conditions of this experiment,the total amount of free amino acids and protein content of tobacco increased significantly with the nitrogen application level,but the contents of free amino acids in different species showed different expression with nitrogen application level,the sensory quality of tobacco was the best in90kg/ha N.

关 键 词：氮素水平 红花大金元 游离氨基酸 蛋白质 感官质量 

分 类 号：S572[农业科学—烟草工业]