H_2O_2对蛇足石杉离体培养物诱变效应的研究  

Studies on H_2O_2 Induced Effect on Huperzia serrata in vitro

作　　者：叶丽婻 涂艺声[1] 黄倩 余晓 袁慧慧 鲁淑敏 YE Li-nan;TU Yi-sheng;HUANG Qian;YU Xiao;YUAN Hui-hui;LU Shu-min(College of Life Science, Jiangxi Normal University, Nanchang 330022, China)

机构地区：[1]江西师范大学生命科学学院,南昌330022

出　　处：《热带亚热带植物学报》2017年第6期587-593,共7页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(81360614;81660597);江西省自然科学基金项目(20132BAB204023)资助~~

摘　　要：为了获得高产石杉碱甲(Huperzine A,Hup A)的蛇足石杉[Huperzia serrata(Thunb.)Trev.]叶状体,对H_2O_2诱变后的叶状体进行了研究。结果表明,诱变后叶状体株系的Hup A含量显著提高,并获得高产株系SH42,其相对生长率和Hup A含量分别达到4499.28%和261.17μg g^(–1) DW,比起始叶状体分别提高了2.35倍和2.43倍;且株系间可溶性蛋白质谱带和SOD同工酶谱均存在差异,经过连续9代培养,变异叶状体可以稳定遗传。因此,H_2O_2对叶状体细胞具有良好的诱变效应,可以用于筛选高产Hup A株系。In order to gain high yield strain of huperzine A(HupA)from Huperzia serrata(Thunb.)Trev.in vitro,the hydrogen peroxide(H2O2)induced thallus was studied.The results showed that the HupA content in induced thallus significantly increased.The strain SH42had highest yield of HupA with relative growth rate and HupA content for4499.28%and261.17μg g–1DW,respectively,which increased2.35times and2.43times of the initial strain,respectively.Moreover,there were significant difference spectral band in SOD isozymes and soluble protein among strains.The variation characteristics were stable after nine generations.Therefore,it was suggested that H2O2had good induced effect on thallus of Huperzia serrata,which could be used for screening high-yield strain of HupA in vitro.

关 键 词：蛇足石杉 叶状体 石杉碱甲 H2O2 可溶性蛋白质 SOD同工酶 

分 类 号：S567.239[农业科学—中草药栽培]