蛹虫草菌侵染柞蚕蛹条件的研究  

Study on Conditions of Antheraea pernyi Pupa Infected by Cordyceps militaris(L.)Link

作　　者：张博[1] 孙宁 孙永健[1] 张颖[1] 路雨 何媛媛 ZHANG Bo;SUN Ning;SUN Yong-jian;ZHANG Ying;LU Yu;HE Yuan-yuan(Biotechnology and Food Engineering Department, Tianjin Tianshi College, Tianjin 301700, China;College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China)

机构地区：[1]天津天狮学院生物与食品工程学院,天津301700 [2]天津农学院农学与资源环境学院,天津300384

出　　处：《天津农学院学报》2017年第4期48-51,共4页Journal of Tianjin Agricultural University

基　　金：天津天狮学院科研项目"柞蚕蛹虫草培育关键技术的研究"(K14004);天津市大学生创新创业训练计划项目"优质桑蚕蛹虫草培育关键技术的研究"(201610859003)

摘　　要：分别从菌种处理、接种方式、寄主三个角度研究了蛹虫草菌侵染柞蚕蛹的条件,结果表明,用添加70%柞蚕蛹浸出汁制作蛹虫草菌的液体培养基对菌种进行活化培养,以注射的方式接种于灭菌后(121℃,20 min)的柞蚕蛹体内,培养出的子实体最好,蛹体完全僵化所需时间缩短至6 d,僵化率及发草率分别提高至98.0%和99.3%。This research was carried out from three aspects,including strain,inoculation and host,to study the conditions of Antheraea pernyi pupa infected by Cordyceps militaris(L.)Link.The result shows that add70%leaching juice of the pupa into medium for activation of strain and inoculate to sterilized(121℃,20min)pupa by injection,can make the fruiting body of Cordyceps militaris(L.)Link achieve the best effect.The time of pupa become rigid can be shortened to6days and the rate of rigidity and germination can be increased to98.0%and99.3%,respectively.

关 键 词：蛹虫草 柞蚕蛹 侵染 

分 类 号：S567.35[农业科学—中草药栽培]