利用16S rDNA扩增子测序技术分析不同品种猪盲肠微生物菌落多样性  

Analysis of the Cecum Microbial Diversity of Different Pig Breeds by Illumina Amplicon Sequencing of 16SrDNA Tag

作　　者：康润敏[1] 李瑶[1] 吕学斌[1] 姬高升 应三成[1] 曾凯[1] 李琰[1] 殷明郁[1] KANG Run-min;LI Yao;LV Xue-bin;YING San-cheng;LI Yan;JI Gao-sheng;ZENG Kai;YIN Ming-yu(Animal Breeding and Genetics Key Laboratrry o f Sichuan Province, Sichuan Animal Science Academy,Chengdu 610066, China;Key Laboratory of Bio resources and Ecoenvironment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064,China)

机构地区：[1]四川省畜牧科学研究院,动物遗传育种四川省重点实验室,成都610066 [2]四川大学生命科学学院,动物疫病防控与食品安全四川省重点实验室,生物资源与生态环境教育部重点实验室,成都610064

出　　处：《中国畜牧兽医》2017年第11期3121-3129,共9页China Animal Husbandry & Veterinary Medicine

基　　金：四川公益性科研院所基本科研项目(SASA2015A03);四川省科技支撑计划(2016NYZ0042、2014NZ0092、16ZC2850);国家生猪产业技术体系(CARS-36);四川省财政运行专项(SASA2014CZYX001)

摘　　要：为了研究中国特有地方猪种丫杈猪、青裕猪和乌金猪盲肠微生物菌落的组成,揭示地方猪种耐粗饲特性的机理,本试验采用16SrDNA扩增子测序技术比较分析了体重90kg左右的丫杈猪、青裕猪和乌金猪盲肠微生物菌群的多样性。结果显示,在门水平上3个品种猪的核心菌群均为拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes),在属的水平上均为普雷沃菌属(Prevotella)和拟杆菌属(Bacteroides);在3个猪种的盲肠中均发现了与降解纤维素相关的细菌,其中纤维杆菌属(Fibrobacter)和梭菌属(Clostridium)在丫杈猪盲肠中的数量显著高于青裕猪和乌金猪(P<0.05),普雷沃氏菌属(Prevotella)在丫杈猪盲肠内的数量显著高于乌金猪(P<0.05),拟杆菌属(Bacteroides)在青裕猪盲肠内的数量显著高于丫杈猪(P<0.05),螺旋体门(Spirochaetes)在青裕猪盲肠内的数量显著高于乌金猪(P<0.05)。结果表明,3个品种猪盲肠内微生物菌群分布的种类相似度高,但分布规律和数量存在显著差异;对与纤维素消化相关菌群的分析表明,丫杈猪对纤维消化的能力是3个品种中最强的,其次是青裕猪,乌金猪最弱。To study the diversity and composition of the bacterial community from cecum samples from Yacha pig,Qingyu pig and Wujin pig,the investigation was designed to reveal mechanism of roughage resistance.Illumina amplicon sequencing of16S rDNA Tag was used to analyze the cecum microbial diversity of Yacha pig,Qingyu pig and Wujin pig at90kg liveweight.The results indicated that the core flora of the three breeds were Bacteroidetes and Firmicutes at the phylum level and Prevotella and Bacteroides at the genus level.Meanwhile bacteria associated with cellulose decomposition were found in all three breeds,in which the number of Fibrobacter and Clostridium in Yacha pig were significantly higher than that in Qingyu pig and Wujin pig(P<0.05),and the number of Prevotella in Yacha pig was significantly higher than that in Wujin pig(P<0.05).The number of Bacteroides in Qingyu pig was significantly higher than that in Yacha pig(P<0.05).The number of Spirochaetes in Qingyu pig was significantly higher than that in Wujin pig(P<0.05).It could conclud that the composition of the cecum bacteria community was similar in above pig breeds,but there were significant differences in distribution and quantity.According to analysis of microbial related to cellulose digestibility,Yacha pig was the strongest among the three breeds,followed by the Qingyu pig and Wujin pig.

关 键 词：丫杈猪 青裕猪 乌金猪 盲肠 微生物多样性 千维素消化能力 

分 类 号：S811.6[农业科学—畜牧学]