组织培养中植物外植体及愈伤组织褐变的研究进展  

Research Progress on Browning of Explant and Callus in Plant Tissue Culture

作　　者：罗阳春 杨云 李仕伟 方芳 王鹏 蒲全波 郑祖平 LUO Yangchun;YANG Yun;LI Shiwei;FANG Fang;WANG Peng;PU Quanbo;ZHENG Zuping(Nanchong Academy of Agricultural Sciences,Nanchong,Sichuan 637000,China)

机构地区：[1]南充市农业科学院,四川南充637000

出　　处：《贵州农业科学》2018年第1期5-10,共6页Guizhou Agricultural Sciences

基　　金：四川省科技厅应用基础计划项目"玉米双单倍体系统化育种研究与利用"(2015JY0247)

摘　　要：植物组织培养是在人工控制和无菌环境条件下,利用适当培养基对植物组织器官、细胞及原生质体进行离体培养,使其再生成细胞或完整植株的一种生物技术。愈伤组织褐变、水质化和污染一直是困扰植物组织培养的3大难题,植物基因型、取样部位、生长年龄、培养基成分及培养条件等不同引起组织褐变的程度也存在差异。为植物组织培养提供参考,对引起植物外植体和愈伤组织褐变的机理、类型、因素及其防治方法等进行归纳总结,并对其今后研究方向进行展望。Plant tissue culture is a biological technique to use suitable media for in vitro culture of tissue organs,cells and protoplasts and then regenerate cells or complete plants under the conditions of artificial control and aseptic environment.Callus browning,vitrification and contamination have always been three problems in plant tissue culture.There is an obvious difference in browning degree among different genotypes,sampling parts,growth age,medium components and culture conditions.The mechanism,types,factors and control methods inducing explant and callus browning are summarized and the future research direction is prospected to provide a reference for plant tissue culture.

关 键 词：愈伤组织 褐变机理 多酚氧化酶 过氧化物酶 苯丙氨酸解氨酶 褐变抑制剂 

分 类 号：S188[农业科学—农业基础科学]