miR-3691-3p靶向调控E2F3/PRDM1对前列腺癌细胞增殖、迁移和侵袭的影响  

miR-3691-3p targeted E2F3/PRDM1 inhibits the biological characteristics of prostate cancer

作　　者：孙莉[1] 牛涵波 徐义英 孙茂民[1] SUN Li;NIU Han-bo;XU Yi-ying;SUN Mao-min(Experimental Animal Center,Soochow University,Suzhou Jiangsu 215123,China)

机构地区：[1]苏州大学实验动物中心,江苏苏州215123

出　　处：《江苏大学学报（医学版）》2018年第1期22-27,共6页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:探讨微小RNA-3691-3p(miR-3691-3p)调控的靶基因E2F3(E2F transcription factor 3)和PR结构域蛋白1(PR domain containing 1,PRDM1)对前列腺癌细胞增殖、迁移与侵袭的影响。方法:在前列腺癌DU145细胞株中,转染不同浓度梯度的miR-3691-3p mimic后,采用实时荧光定量PCR和蛋白质印迹法检测候选靶基因E2F3和PRDM1 mRNA和蛋白表达的变化;人工合成候选靶基因的小干扰RNA(E2F3 siRNA和PRDM1 siRNA)转染入DU145细胞中,采用实时荧光定量PCR检测siRNA在细胞中的转染率;DU145细胞株分别转染阴性对照siRNA、E2F3 siRNA和PRDM1 siRNA后,采用MTT、Transwell、划痕实验检测DU145细胞株增殖、迁移、侵袭能力的变化。结果:随着miR-3691-3p mimic转染浓度增高,在DU145细胞株中E2F3和PRDM1 mRNA的表达量逐渐下降(P<0.01或P<0.05),两个蛋白的表达水平均明显降低(P<0.01)。与阴性对照组相比,转染E2F3 siRNA和PRDM1 siRNA的DU145细胞株中,E2F3和PRDM1在mRNA水平上表达量显著减少(E2F3:t=31,P<0.01;PRDM1:t=10.44,P<0.01)。且转染E2F3 siRNA的DU145细胞增殖、迁移和侵袭能力显著下降(均P<0.01);而转染PRDM1 siRNA的DU145细胞增殖和侵袭能力显著下降(均P<0.01),迁移能力没有明显变化(P>0.05)。结论:在DU145细胞中,低水平的miR-3691-3p通过靶向调控E2F3和PRDM1的增高来增强细胞的生物学特性。To investigate the effects of miR-3691-3p-regulated target genes E2F3and PRDM1on the proliferation,migration and invasion of prostate cancer DU145cell line.Methods:miR-3691-3p mimic with various concentrations was transfected into DU145cell line,RT-PCR and Western blotting were used to detect the expression level of E2F3,PRDM1mRNA and protein.The small interfering RNA scrambled siRNA,E2F3siRNA and PRDM1siRNA were transfected into DU145cells.The expression levels of E2F3,PRDM1mRNA were detected by RT-PCR and MTT,Transwell and scratch tests were used to detect the proliferation,migration and invasion of DU145cell line.Results:The expression of E2F3(P<0.01)and PRDM1(P<0.05)mRNA decreased with the increase of miR-3691-3p mimic transfection concentration in DU145cell line,the expression level of both two protein were decreased(P<0.01).Compared with the negative control group,the expression of E2F3and PRDM1in DU145cell lines transfected with E2F3siRNA and PRDM1siRNA were significantly decreased(E2F3:t=31,P<0.01;PRDM1:t=10.44,P<0.01).The proliferation,migration and invasion of DU145cells transfected with E2F3siRNA,were significantly decreased(all P<0.01).The proliferation,invasion of DU145cells transfected with PRDM1siRNA were significantly decreased(all P<0.01),but the migration ability was not changed(P>0.05).Conclusion:The expression of miR-3691-3p is low in DU145cells,promoting the biological characteristics of cells by targeting E2F3and PRDM1.

关 键 词：前列腺癌 DU145细胞株 miR-3691-3p E2F3 PRDM1 生物学特性 

分 类 号：R737.25[医药卫生—肿瘤]