天麻提取物及其3种主要成分对灰树花产胞外漆酶和菌丝体的影响  

Effect of Rhizoma gastrodiae Extract and Its Three Main Components on Production of Laccase and Mycelial Biomass by Submerged Fermentation of Grifola frondosa

作　　者：芦红云 吴天祥 钟敏[1] 聂文强 LU Hongyun;WU Tianxiang;ZHONG Min;NIE Wenqiang(School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;Mingde College of Guizhou University, Guiyang 550025, China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学明德学院,贵州贵阳550025

出　　处：《食品科学》2018年第6期101-106,共6页Food Science

基　　金：国家自然科学基金地区科学基金项目(31460537)

摘　　要：以天麻提取物为外源添加物,加至灰树花液体深层发酵体系中,通过外源诱导实验,考察天麻提取物对灰树花液体发酵产菌丝体及胞外漆酶的影响,并进一步重点研究起显著影响作用的关键成分。研究表明,天麻提取物及单一成分天麻素(gastrodin,GA)、对羟基苯甲醛(p-hydroxylbenzaldehyde,HBA)、对羟基苯甲醇(p-hydroxybenzyl alcohol,HA)均对灰树花菌丝体和胞外漆酶活力有显著的促进作用。结果显示,当添加3 g/L的天麻醇提物时,对灰树花菌丝体生物量和胞外漆酶活力有最大的促进作用,与对照组相比,分别提高了1.62倍和7.41倍(P<0.05)。同时,利用高效液相色谱对3 g/L天麻醇提物中的GA、HBA、HA含量进行分析,以相应量添加到灰树花发酵体系中。结果表明,HA和HBA均能显著提高灰树花漆酶活力和菌丝体生物量,与添加3 g/L天麻醇提物实验组相比,添加HA使酶活力提高10.25%,生物量增加9.17%。本研究可为中药提取物及成分促进真菌生长和产漆酶提供理论依据。This study investigated the effect of exogenous addition of Rhizoma gastrodiae alcohol extract to the culture medium on the production of mycelial biomass and extracellular laccase by Grifola frondosa in submerged culture.In addition,we identified the role of the key components of the extract,gastrodin(GA),p-hydroxylbenzaldehyde(HBA)and p-hydroxybenzyl alcohol(HA),in affecting the two parameters.These three compounds were quantified by high performance liquid chromatography(HPLC).It was revealed that the R.gastrodiae extract added at proper concentrations,as well as each of its key components at a concentration equivalent to3g/L R.gastrodiae extract,exerted a significant stimulating effect on the growth of mycelial biomass and extracellular laccase activity.By supplementing3g/L R.gastrodiae extract to the medium,the maximum biomass and laccase activity of10.03g/L and106.46U/L,respectively were obtained,1.62and7.41times higher than those of the control group(P<0.05),respectively.Both HA and HBA significantly promoted laccase activity and mycelial production.Compared with3g/L R.gastrodiae extract,the laccase activity and biomass of the experimental group containing HA increased by10.25%and9.17%,respectively.This experiment can provide evidence that R.gastrodiae extract and its purified components can promote fungal growth and laccase activity.

关 键 词：天麻提取物 灰树花 菌丝体生物量 胞外漆酶 关键成分 

分 类 号：Q936[生物学—微生物学]