亚麻籽油和豆油替代鱼油对大黄鱼肝脏和肌肉脂肪酸组成及Δ6Fad基因表达的影响  

THE EFFECTS OF LINSEED OIL AND SOYBEAN OIL ON FATTY ACID COMPOSITION AND Δ6FAD GENE EXPRESSION IN LIVER AND MUSCLE OF LARGE YELLOW CROAKER(LARIMICHTHYS CROCEA)

作　　者：李经奇 李学山 姬仁磊 徐玮[1] 康森艾 艾庆辉[1] LI Jing-Qi;LI Xue-Shan;JI Ren-Lei;XU Wei;KANG Sen-Ai;AI Qing-Hui(The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, China)

机构地区：[1]中国海洋大学教育部海水养殖重点实验室,农业部海水养殖重点实验室,青岛266003

出　　处：《水生生物学报》2018年第2期232-239,共8页Acta Hydrobiologica Sinica

基　　金：国家杰出青年自然科学基金(31525024)资助

摘　　要：为了研究亚麻籽油(LO)和豆油(SO)完全替代鱼油(FO)对大黄鱼(Larimichthys crocea)肝脏和肌肉脂肪酸组成及Δ6Fad(Δ6 Fatty acid desaturase)基因表达的影响。实验用豆油和亚麻籽油替代鱼油制备了3种等氮等脂的精制饲料,在海水浮动网箱中进行了为期10周的养殖实验。实验结果表明:(1)鱼油组的增重率、饲料效率和特定生长率均显著高于亚麻籽油组和豆油组(P<0.05),但对成活率,肝体指数和脏体指数没有显著影响(P>0.05);(2)亚麻籽油和豆油完全替代鱼油显著改变了鱼体肝脏和肌肉的脂肪酸组成,降低了肝脏和肌肉LCPUFA(Long chain-polyunsaturated fatty acid)的相对含量(P<0.05),在亚麻籽油组(LO)和豆油组(SO)中没有显著差异(P>0.05),在各处理组中,肌肉的n-3LC-PUFA的相对含量显著高于肝脏(P<0.05);(3)亚麻籽油和豆油显著上调了肌肉和肝脏中Δ6Fad基因的表达量(P<0.05),其表达量在肝脏中分别升高了7.6和6.5倍,在肌肉中分别上升了2.2和2.8倍。结果表明,在实验条件下亚麻籽油和豆油完全替代鱼油对大黄鱼生长具有不利影响,亚麻籽油和豆油替代鱼油降低了肝脏和肌肉中LC-PUFA的含量,提高了Δ6Fad基因的表达量。To investigate the effects of linseed oil and soybean oil on fatty acid composition and A6Fad gene expressionin liver and muscle of large yellow croaker,three defined diets were made with fish oil(FO),soybean oil(SO)and linseed oil(LO)as fat sources for a10weeks experiment on floating cages.Results indicated that the weight gain rate,feed efficiency ratio and specific growth rate of FO group was significantly higher than LO group and SO group(P<0.05)and no significant difference in survival rate,HSI and VSI(P>0.05)were observed among groups.The fattyacid was changed and the relative content of LC-PUFA(Long chain-polyunsaturated fatty acid)reduced significantly(P<0.05)in liver and muscle when fish oil was replaced by linseed oil and soybean oil,but there was no significant differencebetween linseed oil treatment and soybean oil treatment(Z^O.05).The relative content of n-3LC-PUFA inmuscle was more than that of liver(P<0.05).SO and LO induced the A6Fad expression7.6and6.5times in liver and2.2and2.8times in muscle respectively.Results suggest that the replacement of fish oil by linseed oil and soybean oilhad negative effect on fish performance possibly via lipids metabolism.

关 键 词：大黄鱼 A6Fad 脂肪酸 亚麻籽油 豆油 

分 类 号：S965.3[农业科学—水产养殖]