菠萝蜜种子中总黄酮的提取工艺及其抗氧化性研究  

Total flavonoids extraction and antioxidant activity from jackfruit seed

作　　者：张锦东 王小玉[2] 游淑珠[2] 赵国锋 余以刚 ZHANG Jin-dong;WANG Xiao-yu;YOU Shu-zhu;ZHAO Guo-feng;YU Yi-gang(School of Food Sciences and Engineering,South China University of Technology,Guangzhou 510641,China;Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519000,China;Zhuhai Hexie Agricultural Products Co.,Ltd., Zhuhai 519100,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510641 [2]珠海出入境检验检疫局技术中心,广东珠海519000 [3]珠海市禾协农产品有限公司,广东珠海519100

出　　处：《广东农业科学》2017年第12期136-143,共8页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2016B090918103);珠海进出口公共技术服务平台产学研协同创新计划项目(IETP201601006)

摘　　要：采用四因素三水平正交试验设计优化了菠萝蜜种子中总黄酮的提取工艺。结果表明,在水浴条件下,4个因素对菠萝蜜种子粉的总黄酮提取率的影响大小为:乙醇浓度>料液比>提取温度>提取时间。其中,乙醇浓度对总黄酮提取率影响极显著,料液比、提取温度、提取时间对总黄酮提取率影响显著。水浴条件下的最佳提取工艺为:70%乙醇,60℃水浴提取50 min,料液比1∶20 g/m L,菠萝蜜种子中总黄酮提取率达到3.30%。采用DPPH法和水杨酸法测定菠萝蜜种子中总黄酮粗提液的抗氧化活性,结果表明,总黄酮粗提液浓度在0.6 mg/m L时两种测定方法中自由基的清除率均超过90%,具有较强的抗氧化活性。实际生产中,菠萝蜜种子可作为抗氧化剂的原料来源。The extraction technology of total flavonoids from jackfruit seed powder was studied by four factors three levels orthogonal experiment.The results showed that the effect order of four factors on the extraction rate of total flavonoids from seed powder of jackfruit was:ethanol concentration>liquid ratio>extraction temperature>extraction time.The ethanol concentration had a significant effect on the total flavonoids extraction rate(P<0.01),the ratio of material to liquid,the extraction temperature and extraction time had significant effects on the total flavonoids extraction rate(P<0.05).When the ethanol concentration was70%,the extraction temperature was60℃,the extraction time was50min,the ratio of material to liquid was1∶20,the optimum extraction process under the condition of water bath was reached.The extraction rate of total flavonoids from jackfruit seed powder under the best extraction process conditions reached3.30%.The antioxidant activity of crude flavonoids from jackfruit seed powder was determined by DPPH method and salicylic acid method.The results showed that the scavenging rate of free radicals was more than90%at0.6mg/mL,indicating that the flavonoids from jackfruit seed powder had strong antioxidant activity and had good application prospect.In actual production,the seed of jackfruit can be used as the source of antioxidants.

关 键 词：菠萝蜜种子 正交实验设计 总黄酮 抗氧化性 自由基 

分 类 号：S667.8[农业科学—果树学]