低能氮离子注入驱动DOB的16S rRNA基因突变与进化  

DOB 16S rRNA Gene Mutation and Evolution Driven by Low-Energy Nitrogen Ion Beam Implantation

作　　者：蔡长龙[1] 吕杰[2,3] 毛培宏 马媛[3] CAI Changlong;LYU Jie;MAO Peihong;MA Yuan(Research Center of Ion Beam Biotechnology and Biodiversity,Xi’an Technological University,Xi’an 710021,China;Research Center of Ion Beam Biotechnology,Xinjiang University,Urumqi 830046,China;Laboratory of Molecular Ecology,College of Resource and Environment Sciences,Xinjiang University,Urumqi 830046,China)

机构地区：[1]西安工业大学离子束生物工程与生物多样性研究中心 [2]新疆大学离子束生物技术中心 [3]新疆大学资源与环境科学学院分子生态学研究室

出　　处：《西安工业大学学报》2017年第11期781-786,共6页Journal of Xi’an Technological University

基　　金：国家自然科学基金(11575149;31360131);陕西省社会发展科技攻关项目(2016SF-50)

摘　　要：为了进一步理解低能N+注入在原核微生物系统发育与进化中的作用,文中采用低能氮离子束注入技术和高通量选择性方法获得23株对极端环境适应能力更强的重组DOB菌株,并对23株离子束重组DOB菌株及其原始菌株的16S rRNA基因进行了分子系统学研究.研究结果表明:23株重组DOB菌株的16S rRNA基因均发生了不同程度的碱基突变,累计碱基突变438次,平均突变频率达1.2%,其中保守区内的碱基突变占突变总数的65.30%,突变大部分集中在C7保守区和C4保守区,而C2保守区和V9可变区均未发生碱基突变.通过聚类分析,23株重组DOB菌株的16S rRNA基因聚类后可分为三个进化速度不同的类群.In order to elucidate the mechanism of phylogenetic development and genome evolution on the low energy N+implantation prokaryotic microorganisms,the 16S rRNA gene of 23 recombinant desert oligotrophic bacteria(DOB)and original strain was studied by molecular systematics.The result of multiple comparison shows that different degrees of mutation occurred to the 16S rRNA gene of 23 recombinant DOB strains.The cumulative number of the gene mutation was 438 times and the average mutation rate was 1.2%.65.30%of the mutations occurred in the conserved region.Most of the mutations occurred in the conserved regions C7 and C4,and no mutation in the conserved region C2 and the variable region V9.The results of phylogenetic analysis show that 16S rRNA gene cluster of 23 recombinant DOB strains can be divided into three different groups according to their evolution speed.The results of this research provide the evidence that the low energy ion implantation can drive the genome evolution of prokaryotic microorganisms.

关 键 词：DOB菌株 16SRRNA基因 离子注入 碱基突变 

分 类 号：Q933[生物学—微生物学]