低产桔霉素红曲霉复合诱变育种  

Screening of low-yield citrinin monascus by compound mutation breeding

作　　者：庄晓晓[1] 王钰[1] 李阔阔 沈小瑞[1] ZHUANG Xiao-xiao;WANG Yu;LI Kuo-kuo;SHEN Xiao-rui(Shool of Resources and Environmental Engineering,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601

出　　处：《生物学杂志》2018年第1期104-106,124,共4页Journal of Biology

基　　金：中药材产业技术创新平台(Y01001575)

摘　　要：以通过N+离子束诱变筛选出的高产洛伐他汀红曲霉菌株M50-2为出发菌株,进行紫外诱变,筛选低桔霉素产量菌株。将出发菌株置于30 W紫外灯下30 cm处分别照射30、60、90、120和150 s后,对所得诱变菌株进行液态发酵。采用高效液相色谱法(HPLC)中的荧光检测(λex=331 nm,λem=500 nm)和紫外检测(λ=238 nm)测定发酵产物中的桔霉素和洛伐他汀的含量。结果表明,通过紫外诱变,获得一株优良菌株。其液态发酵的产物中的桔霉素产量为2.15 mg/L,与出发菌株相比桔霉素含量减少了96.6%,而洛伐他汀产量为0.338 mg/m L,与出发菌株相比只减少了16.5%,对该菌株进行传代培养12代,结果表明该菌株具有较好的遗传稳定性。In order to screen out low citrinin yield strain,as a starting strain,the high-yield lovastatin Monascus strain M50-2 was treated by ultraviolet,which was selected by N+ion beam mutagenesis.The starting strain was irradiated at 30 cm under a 30 W UV lamp,and the dose was 30,60,90,120 and 150 s.The contents of citrinin and lovastatin in the fermentation products were determined by high performance liquid chromatography(HPLC)with fluorescence detection(λex=331 nm,λem=500 nm)and ultraviolet detection(λ=238 nm)after the liquid fermentation of the screened mutagenized strain.The results showed that an excellent strain M120-1 was obtained by ultraviolet mutagenesis.The best strain M120-1 yielded 2.15 mg/L citrinin,which was 96.6%lower than the original strain.However,lovastatin production was 0.338 mg/mL,which was only decreased by 16.5%.To examine the genetic stability of M120-1,the strain was continuously sub-cultured to the 12 th generation,showing that the strain had good genetic stability.

关 键 词：红曲霉 桔霉素 洛伐他汀 紫外诱变 复合诱变 

分 类 号：Q933[生物学—微生物学]