液相色谱-串联质谱法测定食品中的4种添加剂  

Determination of 4 Kinds of Additives in Food by Liquid Chromatography-tandem Mass Spectrometry

作　　者：钟茂生 陈舒奕 梁剑 朱品玲 叶洪 肖颖 ZHONG Mao-sheng;CHEN Shu-yi;LIANG Jian;ZHU Pin-ling;YE Hong;XIAO Ying(Ningde Entry-exit Inspection and Quarantine Bureau,Fujian Ningde 352100;Putian Entry-exit Inspection and Quarantine Bureau,Fujian Putian 351100,China)

机构地区：[1]宁德出入境检验检疫局,福建宁德352100 [2]莆田出入境检验检疫局,福建莆田351100

出　　处：《广州化工》2018年第2期104-106,147,共4页GuangZhou Chemical Industry

基　　金：福建出入境检验检疫局科研项目(FK2014-ND001)

摘　　要：建立了食品中安赛蜜、糖精钠、苯甲酸和山梨酸同时检测的液相色谱-串联质谱(LC-MS/MS)检测分析方法。样品经20%甲醇溶液提取,加亚铁氰化钾溶液和乙酸锌溶液沉淀蛋白,加正己烷萃取分离脂肪,以电喷雾电离负离子(ESI-)、多反应检测模式(MRM)进行检测,外标法定量。山梨酸定量下限为0.20 mg/kg,安赛蜜、糖精钠和苯甲酸定量下限均为0.05 mg/kg。方法回收率为84.5%~101.0%,相对标准偏差(RSD)3.0%~9.6%。A method was developed for the simultaneous determination of acesulfame K,saccharin sodium,benzoic acid and sorbic acid in foods using liquid chromatography-tandem mass spectrometry(LC-MS/MS).The sample was extracted with 20%methanol solution,precipitated with potassium ferrocyanide solution and zinc acetate solution,and extracted with n-hexane to separate fat.The samples were determined in the modes of electrospray negative ionization(ESI-)and multiple reaction monitoring(MRM),using external standard method.The lower limit of quantification of sorbic acid was 0.20 mg/kg,and the lower limit of quantification of acesulfame K,saccharin sodium and benzoic acid were 0.05 mg/kg.The recoveries ranged from 84.5%to 101.0%,and the relative standard deviations(RSD)ranged from 3.0%to 9.6%.

关 键 词：液相色谱-串联质谱法 安赛蜜 糖精钠 苯甲酸 山梨酸 食品添加剂 

分 类 号：O657[理学—分析化学]