苦荞提取物对正常小鼠乙醇代谢酶的影响  

Effect of Fagopyrum Tatarium Gaertn Extractive on Alcohol Metabolism in Normal Mice

作　　者：陈志元 鲁晓佳 余小玲 杨跃军 金悠[2] CHEN Zhiyuan;LU Xiaojia;YU Xiaoling;YANG Yuenian;JIN You(Hubei Province Key Laboratory of Traditional Chinese Medicine,Health Food and Safety,Jing Brand Co.,Ltd,Daye 435100,China;Department of Pharmacology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,China)

机构地区：[1]劲牌有限公司中药保健食品与安全湖北省重点实验室,大冶435100 [2]华中科技大学同济医学院药理系,武汉430030

出　　处：《医药导报》2018年第4期438-440,共3页Herald of Medicine

摘　　要：目的观察苦荞提取物灌胃后对小鼠体内乙醇代谢酶的影响,并探讨其可能机制。方法以雄性昆明种小鼠为实验对象,设阴性对照组、小剂量苦荞组、中剂量苦荞组、大剂量苦荞组,2 h后取肝脏组织。一部分肝脏组织用于提mRNA,并用荧光实时定量q PCR技术检测与乙醇和乙醛代谢密切相关的4个酶,即乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、过氧化物酶(POD)、细胞色素P4502E1(CYP2E1)的基因表达水平。另一部分组织则用相关的酶活性试剂盒检测上述4种酶活性的改变。结果小、中、大剂量苦荞提取物不改变小鼠肝脏内ADH的基因表达水平,但中、大剂量组明显增加其活性;小、中、大剂量苦荞提取物均促进小鼠肝脏内ALDH的基因表达水平,且小、中剂量组明显增加其活性;小、中、大剂量苦荞提取物不改变小鼠肝脏内POD基因表达,但大剂量组增加其活性;小、中、大剂量苦荞提取物对肝脏CYP2E1酶的基因表达和活性均无明显影响。结论苦荞可以促进酒精的代谢,加速酒精的排泄,起到解毒作用,并降低对各种细胞器和酶功能损伤的作用。Objective To study the effects of Fagopyrum tatarium Gaertn extractive on metabolism of alcohol and its underlying mechanisms.Methods Male Kunming mice were intragastrically administrated with three different doses(low,median or high)of Fagopyrum tatarium Gaertn extractive or vehicle control,then their liver tissue was collected after 2 h.The gene expression level of alcohol dehydrogenase(ADH),acetaldehyde dehydrogenase(ALDH),peroxidase and cytochrome P4502E1(CYP2E1)in the liver were detected by real-time qPCR,and the enzyme activity was also detected using their respective activity kits.Results Fagopyrum tatarium Gaertn extractive elevated the gene expression level of ALDH but not ADH,peroxidase or CYP450.The activity of ADH was increased after median or high dose treatment,the activity of ALDH was enhanced after low or median dose treatment,while the activity of peroxidase was elevated only by high dose treatment.However,Fagopyrum tatarium Gaertn extractive did not influence the activity of CYP.Conclusion Fagopyrum tatarium Gaertn extractive promotes the metabolism and excretion of alcohol,thus will exerts protective effects on organelles and enzymes associated with cell physiological functions.

关 键 词：苦荞 乙醇脱氢酶 乙醛脱氢酶 过氧化物酶 细胞色素P4502E1 

分 类 号：R285.5[医药卫生—中药学] R575.5[医药卫生—中医学]