妊娠高血压综合征患者胎盘组织中HIF-1α和HSP70的表达及其意义  

Expressions of HIF-1α and HSP70 in placenta tissue of patients with pregnancy-induced hypertension syndrome and their significances

作　　者：蓝永洪[1] 饶朗毓[2] 杨智[1] 黄明媚 牛海艳[1] 齐亚灵[4] LAN Yonghong;RAO Langyu;YANG Zhi;HUANG Mingmei;NIU Haiyan;QI Yaling(Morphological Laboratory,Hainan Medical College,Haikou 571199,China;Department of Pathogenic Biology,Hainan Medical College,Haikou 571199,China;Department of Obstetrics,First Affiliated Hospital,Hainan Medical College,Haikou 570102,China;Department of Histology and Embryology,Hainan Medical College,Haikou 571199,China)

机构地区：[1]海南医学院形态学实验室,海南海口571199 [2]海南医学院病原生物学教研室,海南海口571199 [3]海南医学院第一附属医院产科,海南海口570102 [4]海南医学院组织学与胚胎学教研室,海南海口571199

出　　处：《吉林大学学报（医学版）》2018年第2期346-349,共4页Journal of Jilin University：Medicine Edition

基　　金：海南省科技厅自然科学基金资助课题(814301)

摘　　要：目的:探讨缺氧诱导因子1α(HIF-1α)和热休克蛋白70(HSP70)在妊娠高血压综合征(妊高征)患者胎盘组织中的表达,阐明两者在妊高征患者胎盘组织中表达的临床意义。方法:收集妊高征患者胎盘组织22例(妊高征组)和正常孕妇胎盘组织18例(对组照),采用免疫组织化学法检测2组孕妇胎盘组织中HIF-1α和HSP70的表达情况,分析两者阳性表达率在妊高征患者和正常孕妇胎盘组织中的差异及其在妊高征患者胎盘组织中的关系。结果:妊高征组患者和对照组正常孕妇胎盘组织中HIF-1α阳性表达率分别为81.8%和38.9%,妊高征组患者胎盘组织中HIF-1α阳性表达率明显高于对照组(χ2=7.785,P=0.005);妊高征组患者和对照组正常孕妇胎盘组织中HSP70阳性表达率分别为90.9%和55.6%,妊高征组患者胎盘组织中HSP70阳性表达率明显高于对照组(χ2=6.599,P=0.010)。妊高征组患者中18例HIF-1α阳性表达的胎盘组织全部HSP70表达阳性(100.0%),而4例HIF-1α阴性表达的胎盘组织有2例HSP70表达阴性(50.0%),两者表达存在明显的正相关关系(r=0.671,P=0.001)。结论:妊高征患者胎盘组织中HIF-1α和HSP70呈高表达,且两者的表达呈正相关关系。Objective:To investigate the expressions of hypoxia-inducible factor-1α(HIF-1α)and heat shock protein 70(HSP70)in the placenta tissue of the patients with pregnancy-induced hypertension syndrome(PIH),and to elucidate the clinical significances of their expressions in the placenta tissue of the patients with PIH.Methods:Twenty-two cases of placenta tissue of the PIH patients(PIH group)and eighteen cases of placenta tissue of the normal pregnant women(control group)were selected.The expressions of HIF-1αand HSP70 were detected by immunohistochemical method.The differences of potive expressions rates of HIF-1αand HSP70 in placenta tissue of the PIH patients and the normal pregnant women and their relationships in placenta tissue of the PIH patients were analyzed.Results:The positive expression rate of HIF-1αin placenta tissue of the patients in PIH group(81.8%)was higher than that of the normal pregnant women in control group(38.9%)(χ2=7.785,P=0.005),and the positive expression rate of HSP70 in placenta tissue of the patients in PIH group(90.9%)was higher than that of the normal pregnant women in control group(55.6%)(χ2=6.599,P=0.010).Eighteen cases of placenta tissue with HIF-1αpositive expression in the PIH patients had HSP70 positive expression(100.0%);among four cases of placenta tissue with HIF-1αnegative expression,two cases had HSP70 negative expression(50.0%);their expressions in placenta tissue of the PIH patients had positive correlation(r=0.671,P=0.001).Conclusion:The expressions of HIF-1αand HSP70 in placenta tissue of the PIH patients are higher,and they have positive correlation.

关 键 词：妊娠高血压综合征 胎盘组织 缺氧诱导因子1Α 热休克蛋白70 相关性分析 

分 类 号：R714[医药卫生—妇产科学]