抑制DNA-PKcs对放射治疗后食管鳞癌细胞系EC109自噬蛋白表达和增殖的影响  

Effects of blocking DNA-PKcs on autophagy protein expression and proliferation in esophageal squamous cell carcinoma cell line EC109 after irradiation

作　　者：孙佳[1] 赵晓静[1] 袁翎 李雨濛[1] 李南[1] 张中冕[1] 王豪勋[1] 韩娜[1] 马军[3] 王健[1] SUN Jia;ZHAO Xiaojing;YUAN Ling;LI Yumeng;LI Nan;ZHANG Zhongmian;WANG Haoxun;HAN Na;MA Jun;WANG Jian(Department of Oncology,Second Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450014,China;Affiliated Tumor Hospital of Zhengzhou University/Henan Provincial Tumor Hospital,Zhengzhou,Henan 450008,China;Department of Clinical Laboratory,Second Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450014,China)

机构地区：[1]郑州大学第二附属医院肿瘤科,河南郑州450014 [2]郑州大学附属肿瘤医院/河南省肿瘤医院,河南郑州450008 [3]郑州大学第二附属医院检验科,河南郑州450014

出　　处：《重庆医学》2018年第9期1158-1160,1164,共4页Chongqing medicine

基　　金：河南省科技厅科技攻关项目(152300410164)

摘　　要：目的研究抑制DNA-PKcs对放射照射(X-Ray)后食管鳞癌细胞EC109自噬蛋白表达和增殖的影响。方法用NU7441抑制DNA-PKcs;放射照射处理食管鳞癌细胞EC109;实验共分为4组(对照组、NU7441组、X-Ray组、X-Ray+NU7441组)。蛋白免疫印迹(Western blot)法检测自噬蛋白Beclin-1及LC3B的表达;流式细胞术检测凋亡;四甲基偶氮唑盐(MTT)法检测细胞增殖情况。结果 p-DNA-PKcs在NU7441处理食管鳞癌细胞EC109后表达降低,X-Ray照射后表达升高。与未处理细胞(对照组)相比,X-Ray+NU7441组Beclin-1及LC3B均表达升高,与X-Ray组相比,X-Ray+NU7441组Beclin-1表达升高。与对照组相比,X-Ray组及X-Ray+NU7441组凋亡率明显增加,差异有统计学意义(P<0.05)。与X-Ray组相比,X-Ray+NU7441组细胞凋亡率明显增加。MTT结果显示,与对照组相比,X-Ray组及X-Ray+NU7441组增殖明显被抑制,差异有统计学意义(P<0.05)。结论 NU7441抑制EC109细胞DNA-PKcs蛋白表达后,可促进肿瘤细胞自噬蛋白Beclin-1和LC3B表达,促进凋亡、抑制细胞增殖。Objective To study the effect of DNA-PKcs blocking on the expression of autophagic proteins,and proliferation of esophageal squamous cell carcinoma cells(EC109)after irradiation(X-Ray).Methods NU7441 was used to inhibit DNA-PKcs and X-Ray radiation treatment were used to treat EC109 cells.Th experiment was divided into 4 groups,including control group,NU7441 group,X-Ray group,X-Ray+NU7441 group.The expressions of autophagy protein Beclin-1 and LC3B were detected by Western blot.Apoptosis was detected by flow cytometry.MTT assay was used to detect cell proliferation.Results The expression of p-DNA-PKcs in EC109 cells was decreased after NU7441 treatment and increased after X-ray irradiation.Compared with untreated cells(control group),the expressions of both Beclin-1 and LC3B in X-Ray+NU7441 group were increased.Compared with the X-Ray group,the expression of Beclin-1 in the X-Ray+NU7441 group was increased.Compared with the control group,the apoptotic rate of EC109 cells in the X-Ray group and X-Ray+NU7441 group was significantly increased,the difference was statistically significant(P<0.05).Compared with the X-Ray group the apoptosis rate in the X-Ray-NU7441 group was significantly increased.The MTT results showed that compared with the control group,the proliferation in the X-Ray group and X-Ray+NU7441 group was significantly inhibited,the difference was statistically significant(P<0.05).Conclusion NU7441 inhibits the expression of DNA-PKcs protein in EC109 cells,which could promote the expressions of autophagy protein Beclin-1 and LC3B,promotes apoptosis and inhibits cell proliferation.

关 键 词：DNA-PKCS 自噬蛋白 细胞增殖 食管肿瘤 癌 鳞状细胞 

分 类 号：R735.1[医药卫生—肿瘤]