伴生介质漆酶的生产优化及其酶学特性研究  

Production and Characterization of the Laccase with an Endogenous Mediator

作　　者：冯年捷 傅瑜[3] 王传槐 FENG Nian-jie;FU Yu;WANG Chuan-huai(Hubei Provincial Key Laboratory of Green Materials for Light Industry,Hubei University of Technology,Wuhan 430068;Jiangsu Key Laboratory of Pulp and Paper Science and Technology,Nanjing Forestry University,Nanjing 210037;School of Chemical and Environmental Engineering,Jiangsu University of Technology,Changzhou 213001)

机构地区：[1]湖北工业大学绿色轻工材料湖北省重点实验室,湖北武汉430068 [2]南京林业大学江苏省制浆造纸科学与技术重点实验室,江苏南京210037 [3]江苏理工学院化学与环境工程学院,江苏常州213001

出　　处：《纤维素科学与技术》2018年第1期8-16,共9页Journal of Cellulose Science and Technology

基　　金：湖北工业大学博士科研启动基金

摘　　要：为了缓解人工合成漆酶介质价格昂贵和环境污染的问题,利用血红密孔菌NFZH-1制备伴生介质漆酶,以实现漆酶介质体系(LMS)的工业化应用。通过优化菌株NFZH-1液态培养基和发酵条件,提高了伴生介质漆酶产量,并揭示了其酶学特性。结果表明:血红密孔菌NFZH-1为伴生介质漆酶高产菌株,经产酶优化,漆酶活力提高了91%,达26.1 U/m L。伴生介质漆酶在50～85℃和p H2.5～4.5范围内具有较高的漆酶活力,在<60℃和p H5.0～7.0范围内具有较好的漆酶稳定性,属耐热型漆酶。高产、稳定的伴生介质漆酶对LMS的工业化应用具有重要意义。The laccase with an endogenous mediator was prepared with Pycnoporus sanguineus NFZH-1,to alleviate the cost and environmental problems of artificial mediator.This will be developed for the industrial application of laccase mediator system(LMS).In the present study,the production of the laccase with an endogenous mediator was improved by the optimizing of liquid medium and fermentation conditions.Its enzymatic characteristics were also revealed.The results showed that P.sanguineus NFZH-1 was a high-yield laccase-producing fungus.The laccase activity was increased by 91%,and reached to 26.1 U/mL by means of the above optimizing.The reaction of the laccase would like to happen at temperatures 50～85℃and pH2.5～4.5.The laccase was found to be stable at temperatures below 60℃and pH5.0～7.0.The thermostability of the laccase was also identified.The high yield of the thermostability laccase with an endogenous mediator will be significant for the industrial application of LMS

关 键 词：漆酶 血红密孔菌 伴生介质 液态培养基 发酵条件 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]