过氧化氢降解水不溶性条斑紫菜多糖  

Degradation of water-insoluble polysaccharide from Porphyra yezoensis by hydrogen peroxide

作　　者：王灵昭[1,2] 史辰娟 汪维喜 苏琳 王纪丰[1] 邱春江 舒留泉[1] 姚兴存[1] WANG Ling-zhao;SHI Chen-juan;WANG Wei-xi;SU Lin;WANG Ji-feng;QIU Chun-jiang;SHU Liu-quan;YAO Xing-cun(Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Life and Fisheries,Huaihai Institute of Technology, Lianyungang 222005,China;Lianyungang Xishu Material Co.,Ltd.,Lianyungang 222042,China)

机构地区：[1]淮海工学院江苏省海洋生物技术重点实验室/海洋生命与水产学院,江苏连云港222005 [2]连云港西墅物产有限责任公司,江苏连云港222042

出　　处：《食品与发酵工业》2018年第3期204-208,共5页Food and Fermentation Industries

基　　金：连云港市科技项目(CXY1419);连云港市科技项目(CN1503)

摘　　要：研究了以过氧化氢降解水不溶性条斑紫菜多糖的影响因素,并用液相色谱、红外光谱、紫外光谱法对降解产物进行检测得到蛋白质含量和总糖含量。结果表明,多糖降解的最适条件为:过氧化氢质量分数为1.8%,温度60℃,时间6.0 h;产物包含3种低聚糖组分,可能为双糖、双糖衍生物或聚合度不低于3的低聚糖;产物主要是β-糖苷键连接的吡喃型低聚糖,具有硫酸基和3,6-内醚半乳糖结构;产物含有蛋白质2.12%,总糖93.66%。The degradation products of polysaccharide can overcome its limitations including poor solubility and low bioavailability.In this work,several problems of the degradation of water-insoluble polysaccharide from Porphyra yezoensis by hydrogen peroxide were investigated,including the influence factors and the high performance liquid chromatography,infrared spectrum;ultraviolet spectrum,protein content and total sugar content of products.The optimal conditions of polysaccharide degradation were determined to be hydrogen peroxide concentration 1.8%,temperature 60℃,and time 6.0 h.The products contained three oligosaccharides,which might be disaccharide,disaccharide derivative,or oligosaccharides with polymerization degree≥3.The main products were pyranose oligosaccharides withβ-glucosidic bonds,sulphuric groups,and 3,6-anhydrogalactose structures,and the products contained 2.12%protein and 93.66%total sugar.

关 键 词：条斑紫菜 水不溶性多糖 过氧化氢 降解 红外光谱 

分 类 号：TS254.58[轻工技术与工程—水产品加工及贮藏工程]