微生物葡萄糖氧化酶的研究进展  

Research advance of glucose oxidase from microorganisms

作　　者：李蓉[1,2] 张庆芳 迟乃玉[1,2] LI Rong;ZHANG Qingfang;CHI Naiyu(College of Life Science and Biotechnology,Dalian University,Dalian 116622,China;Liaoning Technology of Marine M icrobiological Engineering Research Center,Dalian 116622,China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]辽宁省海洋微生物工程技术研究中心,辽宁大连116622

出　　处：《中国酿造》2018年第3期1-5,共5页China Brewing

基　　金：国家高技术研究发展计划‘863计划’项目(2007AA021306)

摘　　要：葡萄糖氧化酶(EC 1.1.3.4)作为一种高特异性氧化还原酶,能够催化β-D-葡萄糖转化为葡萄糖酸,并产生过氧化氢(H_2O_2),在医疗检测、食品、生物燃料电池、畜牧养殖等领域发挥着极其重要的作用。该文从微生物来源、克隆表达、发酵优化、分离纯化及应用方面,阐述了葡萄糖氧化酶的研究进展,以期为葡萄糖氧化酶的开发和应用提供参考。Glucose oxidase(EC 1.1.3.4),as a kind of highly specific oxidoreductase,can catalyze P-D-glucose into gluconic acid and H2O2.Glucose oxidases play an extremely important role in medical detection,food,bio-fuel,livestock breeding and other fields.This article expounded the research progress of glucose oxidase,from the aspects of microbial sources,cloning and expression,fermentation optimization,separation,purification and application,in order to provide reference for the development and application of glucose oxidase.

关 键 词：葡萄糖氧化酶 微生物 克隆表达 分离纯化 应用 

分 类 号：Q556.1[生物学—生物化学]