马杜拉放线菌(Actinomadura sp.)FXJ1.344发酵产物的分离鉴定与活性分析  

Separation, identification and activity analysis of fermentation products derived from Actinomadura sp. FXJ1.344

作　　者：余成 刘宁[2] 张志敏[3] 黄英[2] 岳昌武[1] YU Cheng;LIU Ning;ZHANG Zhimin;HUANG Ying;YUE Changwu(Key Laboratory of Microbial Resources and Drug Development in Guizhou Province of Zunyi Medical College,Central Laboratory of Third Affiliated Hospital of Zunyi Medical College,Zunyi 563003,China;State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100020,China;Department of Medical Genetics,Zunyi Medical College,Zunyi 563003,China)

机构地区：[1]遵义医学院第三附属医院中心实验室遵义医学院贵州省微生物资源及药物开发特色重点实验室,贵州遵义563003 [2]中国科学院微生物研究所微生物资源前期开发国家重点实验室,北京100020 [3]遵义医学院医学遗传学教研室,贵州遵义563003

出　　处：《中国酿造》2018年第3期33-37,共5页China Brewing

基　　金：中科院微生物研究所微生物资源前期开发国家重点实验室开放课题(SKLMR-20160601);贵州省科学技术基金(黔科合基础(2017)1218);遵义市科技局基金项目(遵市科合社字(2017)27号;遵市科合社字(2017)36号;遵市科合社字(2017)53号);贵州省卫计委科学基金项目(gzwjkj-2017-1-048)

摘　　要：分离鉴定来源于江西酸性红壤中的马杜拉放线菌(Actinomadura)FXJ1.344所产生的次级代谢产物,并对其中生物活性物质进行分析。采用无水乙醇提取放线菌FXJ1.344发酵菌丝体,粗提物依次用硅胶柱层析法和凝胶柱层析法分离纯化,高效液相色谱法进行制备,并采用超高效液相色谱串联质谱法和核磁共振分析鉴定化合物结构。最终得到2个纯化物,分别为6-hydroxytetrangulol和tetrangulol。用滤纸片扩散法和对倍稀释法检测其抑菌活性,结果表明这2个纯化物均有较好的抗藤黄微球菌(Micrococcus luteus)活性,其最小抑菌浓度分别为3.90μg/m L和15.6μg/m L。The secondary metabolites produced by Actinomadura sp.FXJ1.344 that derived from acid red soil in Jiangxi were separated and identified,and the bioactive substances were obtained and analyzed.With anhydrous ethanol extraction of strain FXJ1.344 for mycelium fermentation,the crude extract was isolated and purified by silica gel column chromatography and molecular sieve chromatography.The pure compounds were prepared by HPLC and identified by UPLC-MS/MS and nuclear magnetic resonance spectroscopy.Finally,two pure compounds were obtained and identified as 6-hydroxytetrangulol and tetrangulol,respectively.the antibacterial activities of two compounds were detected with filter paper diffusion method and dilution method.The results showed that two compounds had good resistance to Micrococcus luteus,and the minimum inhibitory concentrations were 3.9 0 ug/ml and 15.6ug/ml,respectively.

关 键 词：马杜拉放线菌 发酵培养 次级代谢产物 分离鉴定 抑菌活性 

分 类 号：R98[医药卫生—药品]