HPLC-ELSD法同时测定酵母发酵液中的多元醇  

Simultaneous determination of polyhydric alcohols in yeast fermentation broth by HPLC-ELSD

作　　者：王晓丹[1,2] 白晓燕 朱国军 雷安亮 陈美竹 邱树毅[1,2] WANG Xiaodan;BAI Xiaoyan;ZHU Guojun;LEI Anliang;CHEN Meizhu;QIU Shuyi(Guizhou Provincial Key Laboratory of Fermentation Engineering and Biological Pharmacy,Guiyang 550025,China;School of Liquor-making and Food Engineering,Guizhou University,Guiyang 550025,China;GuizhouZhenjiu Wine Co.,Ltd.,Zunyi 563000,China)

机构地区：[1]贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]贵州大学酿酒与食品工程学院,贵州贵阳550025 [3]贵州珍酒酿酒有限公司,贵州遵义563000

出　　处：《中国酿造》2018年第3期162-165,共4页China Brewing

基　　金：贵州省科技支撑计划项目(黔科合支撑[2016]2340);贵州省工业攻关项目(黔科合GZ字[2014]3012);贵州省重大专项项目(黔科合重大专项字[2015]6012)

摘　　要：建立高效液相色谱-蒸发光散射检测器法(HPLC-ELSD)测定酵母菌株发酵液中多元醇含量的方法。采用Agilent Hi-plex Ca色谱柱(7.7 mm×300 mm,8μm),以纯水为流动相,流速0.5 m L/min,蒸发光散射检测器(ELSD)为检测器;柱温为70℃,进样量为4μL。结果表明,5种多元醇在0.8~6.0 mg/m L质量浓度范围内与峰面积呈良好的线性关系(相关系数R均≥0.996);精密度实验结果相对标准偏差(RSD)均<5%,平均回收率为96.73%~100.46%,表明该方法灵敏度高,重现性良好,准确度高,可用于发酵液中5种多元醇的检测。以酱香型白酒酿造过程中大曲和酒醅分离的酵母为研究对象,运用该方法在酵母发酵液中检测到5种多元醇。The method for determination of polyhydric alcohols content in yeast fermentation broth was established by HPLC-ELSD.The samples were separated on Agilent Hi-plexCa chromatographic column(7.7 mmx300 mm,8 pm),with pure water as mobile phase at a flow rate of 0.5 ml/min.The evaporative light-scattering detector(ELSD)was used as detector.Column temperature was 70^and the injection volume was 4 pl.The results showed that 5 polyhydric alcohols had a good linear relationship with peak area in the range of 0.8-6.0 mg/ml(R^0.996).The relative standard deviation(RSD)of precision experiment results was less than 5%,the recovery rate was 96.73%-100.46%.The result indicated that the method showed high sensitivity,good reproducibility and high accuracy,and it could be used for the detection of 5 kinds of polyhydric alcohols in fermentation broth.Using the yeast separated^om Daqu and fermented grains during the brewing process of Moutai-flavor Baijiu as the research object,5 polyhydric alcohols in yeast fermentation broth could be detected by the method.

关 键 词：酵母 高效液相色谱-蒸发光散射检测器法 多元醇 酵母发酵液 

分 类 号：O657.7[理学—分析化学]