热提法提取小黄鱼鱼鳞胶原蛋白工艺优化  

Optimization of Heating Extraction Technology of Collagen from Fish Scale of Small Yellow Croaker

作　　者：苏凤贤[1] 张百刚[2] SU Fengxian;ZHANG Baigang(Wenzhou Vocational College of Science and Technology,Wenzhou 325006,China;College of Life science and engineering,Lanzhou University of Technology,Lanzhou 730050,China)

机构地区：[1]温州科技职业学院,浙江温州325006 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《温州科技职业学院学报》2018年第1期39-45,共7页Journal of WenZhou Vocational College of Science And Technology

基　　金：温州市公益性科技计划项目(N20l40(M3);甘肃省高等学校科研项目(2016B-024)

摘　　要：[目的]以小黄鱼鱼鱗为原料,分析小黄鱼鱼鱗的化学成分;采用热水浸提法提取小黄鱼鱼鱗中的胶原蛋白,测定其理化性质;[方法]通过单因素和正交实验探讨热提法提取胶原蛋白的最佳工艺条件;[结论]热提法的最佳工艺条件为料液比1:13,热提温度66.5°C,热提时间246min;热提法提取的鱼鱗胶原蛋白的起泡性、吸油性均优于酶法提取的鱼鱗胶原蛋白,而两种方法制取的鱼鱗胶原蛋白的气泡稳定性和纯度无显著性差异。[Objective]In this study,by using fish scale of small yellow croaker as raw materials,analysis of the chemical composition of the fish scales and collagen were conducted.The collagen was extracted from fish scale of small yellow croaker by heating extraction to determine their physicochemical properties.[Method]Through single factor and orthogonal experiments,the optimal conditions were discussed by employing heating extraction method.[Conclusion]The optimal conditions using heating extraction are as follows:solid-liquid ratio is 1:13,extraction temperature at 66.5 XI,extraction time is 246 min.Besides,the foamability and oil absorbency of collagen by heating extraction were better than enzymatic extraction,while there were no significant difference of the foaming stability and purity of collagen between heating extraction and enzymatic extraction methods.

关 键 词：鱼鳞 热水浸提 胶原蛋白 

分 类 号：Q814[生物学—生物工程]