ALDH1蛋白表达与胶质瘤干细胞体外分化相关性的研究  

Relationship between ALDH1 expression and cell differentiation of glioma stem cells in vitro

作　　者：王鹏[1] 宋伟正[1] 毛庆[2] 陈米娜[3] 刘艳辉[2] Wang Peng;Song Weizheng;Mao Qing;Chen Mina;Liu Yanhui(Department of Neurosurgery,the Fifth People's Hospital of Chengdu,Chengdu 611130,China;Department of Neurosurgery West China Hospital, Sichuan University, Chengdu 610041, China;State Key Laboratory of the Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]成都市第五人民医院神经外科,611130 [2]四川大学华西医院神经外科,成都610041 [3]四川大学华西医院生物治疗国家重点实验室

出　　处：《中华细胞与干细胞杂志（电子版）》2018年第1期17-21,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：国家自然科学基金资助项目(30873007)

摘　　要：目的探讨乙醛脱氢酶1(ALDH1)蛋白表达与胶质瘤干细胞体外分化相关性。方法将原代培养的胶质瘤干细胞分为分化组与对照组,分化组细胞使用10﹪的胎牛血清诱导,对照组细胞继续在无血清环境中培养,通过免疫荧光细胞化学染色和Western blot观察两组细胞ALDH1蛋白的表达情况,分别使用Wilcoxon符号秩检验和配对样本t检验分析两组ALDH1阳性细胞率和相对蛋白含量的差异。结果分化组细胞呈完全贴壁生长,异型性明显,对照组细胞呈团状聚集,形态较为均一。两组的ALDH1阳性细胞率分别为:分化组18.78﹪±6.03﹪,对照组81.23﹪±3.19﹪;ALDH1相对蛋白含量分别为:分化组0.035±0.009,对照组0.390±0.108。两组的阳性细胞率和相对蛋白含量比较差异具有统计学意义(Z=-2.666,P=0.008;t=-10.637,P=0.000)。结论本实验通过半定量研究进一步证实了在体外培养状态下,ALDH1主要存在于较为原始的肿瘤细胞中,分化后几乎不表达,提示ALDH1作为可能的胶质瘤干细胞标志物仍有进一步研究的价值。Objective To evaluate the relationship between the expression of ALDH1 protein and cell differentiation of glioma stem cells in vitro.Methods The primary cultured glioma stem cells were divided into the differentiation group and the control group.The cells in the differentiation group were induced by 10﹪fetal bovine serum,and the cells in the control group were cultured in the serum-free medium.Immunofluorescence staining and Western blot were used to observe protein expression of ALDH1,and differences of positive cells and relative protein content between the two groups were analyzed by Wilcoxon signed-rank test and paired sample t test.Results The cells in the differentiation group were multiform and completely adhered,and in the control group they were clustered together with uniform morphology.The rate of ALDH1 positive cells in the differentiation group and control group were 18.78﹪±6.03﹪and 81.23﹪±3.19﹪respectively,and relative protein content in the two groups were 0.035±0.009 and 0.390±0.108 respectively.Statistical analysis showed that there was significant difference of positive cell rate and relative protein content between the two groups(Z=-2.666,P=0.008;t=-10.637,P=0.000).Conclusions This semi quantitative study further confirmed that ALDH1 was mainly existed in relatively primitive tumor cells and almost not expressed after differentiation,suggesting that ALDH1 is a potential glioma stem cell marker.

关 键 词：乙醛脱氢酶 胶质母细胞瘤 肿瘤干细胞 细胞分化 

分 类 号：R739.4[医药卫生—肿瘤]