响应面法优化超声辅助提取花椒叶黄酮工艺研究  

Study on the Optimum Technology of Ultrasonic-assisted Extraction of Leaves of Zanthoxylum by Response Surface Method

作　　者：崔鹏[1,2] 高元栋 李玉文 袁岐山 CUI Peng;GAO Yuan-dong;LI Yu-wen;YUAN Qi-shan(Department of Environment and Chemical Engineering,Yellow River Conservancy Technical Institute,Kaifeng 475004,Henan,China;Key Laboratory of Green Coating Materials of Kaifeng City,Kaifeng 475004,Henan,China;College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004,Henan,China;Henan Tobacco Industry Co.,Ltd.,Zhengzhou 450000,Henan,China)

机构地区：[1]黄河水利职业技术学院环境与化学工程系,河南开封475004 [2]开封市绿色涂层材料重点实验室,河南开封475004 [3]河南大学化学与化工学院,河南开封475004 [4]河南中烟工业有限责任公司技术中心,河南郑州450000

出　　处：《食品研究与开发》2018年第8期53-60,共8页Food Research and Development

摘　　要：研究超声波辅助提取花椒叶中黄酮的工艺,并考察了其抗氧化活性。根据单因素试验结果,采用响应面法对该提取工艺进行优化。结果显示:最佳试验条件为乙醇体积百分数60%、液料比20∶1(mL/g)、超声时间20 min、超声温度60℃。此时黄酮的提取率为10.13 mg/g,而模型预测黄酮的提取量为10.18 mg/g,理论预测值与接近试验结果接近。对提取物进行抗氧化测定,发现对·OH清除率为57.21%,ABTS^+·清除率为47.32%,证明其具有较强的抗氧化能力。Flavonoids were extracted from the leaves of Zanthoxylum with the ultrasonic-assisted method and its antioxidant activity was investigated.According to the results of single factor test,the response surface method was used to optimize the extraction process.According to the experiment results,the optimum test conditions were as follows:the percentage of ethanol volume was 60%,the liquid-solid ratio was 20∶1(mL/g),the ultrasonic time was 20 min,and the ultrasonic temperature was 60℃.Under the best conditions,the extraction rate of flavonoids was 10.13 mg/g and it was close with the value predicted from the theoretical model which was 10.18 mg/g.Based on the results of antioxidant activity,the clearance rate of·OH was 57.21%and the clearance rate of ABTS+·was 47.32%,which proved that it had strong antioxidant capacity in vitro.

关 键 词：花椒叶 超声辅助提取 黄酮 响应面法 抗氧化活性 

分 类 号：TQ28[化学工程—有机化工]