趋化因子受体CCR1及CCR5在胃癌中的表达及临床意义  

Expression of chemokine receptors CCR1 and CCR5 in gastric carcinoma and its clinical significance

作　　者：韩璐 周炳娟[1] 孙吉瑞 赵文明[1] 李春辉[2] 张金库[1] Han Lu;Zhou Bingjuan;Sun Jirui(Department of Pathology,the First Central Hospital of Baoding,Baoding 071000,China)

机构地区：[1]保定市第一中心医院病理科,071000 [2]承德医学院附属医院病理科

出　　处：《中华保健医学杂志》2018年第2期105-108,共4页Chinese Journal of Health Care and Medicine

摘　　要：目的探讨胃癌组织和癌旁正常组织中趋化因子受体CCR1和CCR5的表达及分布情况,并分析其与临床病理特征的相关性,同时研究上述两种因子在胃癌组织中的表达是否存在联系。方法采用免疫组织化学和Western blot的实验研究方法,检测胃癌组织及其癌旁正常组织中CCR1及CCR5的表达水平,找到两者与各自临床病理特点的相关性,及其表达的相互关联性。结果免疫组织化学结果显示,胃癌组织中CCR1的检出阳性率为52%,癌旁正常组织中CCR1的阳性率为25%,两种组织中CCR1的阳性表达率差异有统计学意义(P<0.05);胃癌组织中CCR5的阳性率为60%,癌旁正常组织中CCR5的阳性率为28%,差异有统计学意义(P<0.05)。Western blot结果显示,CCR1和CCR5在胃癌组织中的相对表达量分别为(2.134±0.193)和(2.736±0.125),但二者在癌旁正常组织中的相对表达量为(1.378±0.047)和(0.971±0.027),差异有统计学意义(P<0.05)。CCR1的表达水平与患者的性别、年龄、分化程度及浸润深度均无关(P>0.05),但其与肿瘤分化程度及淋巴结的转移具有相关性(P<0.05);CCR5在组织中的表达与胃癌患者的性别、年龄及分化程度无相关性(P>0.05),与浸润深度及淋巴结转移呈正相关(P<0.05)。CCR1与CCR5的表达成正相关(P<0.05)。结论组织中CCR1及CCR5的表达水平升高可能与胃癌的发生、发展具有关联性,通过对CCR1及CCR5的检测,可能对临床诊断胃癌具有一定的提示作用。Objective To investigate the expression and distribution of chemokine receptors CCR1 and CCR5 in gastric carcinoma and adjacent normal tissues,to analyze their correlation with clinicopathological features,and to investigate whether the expression of these two factors in gastric cancer tissues correlate.Methods The expression of CCR1 and CCR5 in gastric cancer tissues and adjacent normal tissues was detected by immunohistochemical methods and by western blot.The purpose was to record and analyze expression levels,to find the correlation between these and pathological characteristics,and to analyze the correlation.Results Immunohistochemistry showed that the positive rate of CCR1 in gastric cancer tissues was 52%(31/60).The positive rate of CCR1 in normal tissues was 25%(15/60).The positive rate of CCR1 in gastric cancer tissues was significantly higher than that of adjacent normal tissues(P<0.05).The positive rate of CCR5 in gastric cancer tissues was 60%,and the positive rate of CCR5 in adjacent normal tissues was 28%.The positive rate of CCR5 in the two tissues was very different and statistically significant(P<0.05).Western blot results showed that the relative expression levels of CCR1 and CCR5 in gastric cancer tissues were 2.134±0.193 and 2.736±0.125,respectively,but the relative expression levels of CCR1 and CCR5 were 1.378±0.047 and 0.971±0.027 in normal tissues,respectively(P<0.05).The expression level of CCR1 was not correlated with gender,age,differentiation degree or depth of invasion(P>0.05),but it was correlated with tumor differentiation and lymph node metastasis(P<0.05).The expression of CCR5 in tissues was not correlated with sex,age and differentiation of gastric cancer(P>0.05)but was positively correlated with invasion depth and lymph node metastasis(P<0.05).CCR1 was positively correlated with CCR5 expression(P<0.05).Conclusion The expression of CCR1 and CCR5 may be associated with the occurrence and development of gastric cancer.It may be helpful to diagnose gastric cancer using CCR1 and

关 键 词：胃癌 CCR1 CCR5 表达 临床意义 

分 类 号：R735.2[医药卫生—肿瘤]