忍冬根的正丁醇萃取物化学成分研究  

Chemical Constituents in n-Butanol Extract fromLonicera japonica Thunb.Roots

作　　者：王丹[1,2] 耿岩玲[1] 陈燕平 杨鹏 李圣波 于金倩 WANG Dan;GENG Yan-ling;CHEN Yan-ping;YANG Peng;LI Sheng-bo;YU Jin-qian(Key Laboratory of TCM Quality Control Technology,Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;College of Food Science and Engineering,Shandong Agricultural University,Taian 271018,China;Yantai Entry-Exit Inspection and Quarantine Bureau,Yantai 264000,China;Jinan Senkang Sanfeng Biological Engineering Co.,Ltd.,Jinan 250014,China;Qinghai Yate Biological Technology Co.,Ltd.,Xining 810000,China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省分析测试中心山东省中药质量控制技术重点实验室,山东济南250014 [2]山东农业大学食品科学与工程学院,山东泰安271018 [3]烟台出入境检验检疫局,山东烟台264000 [4]济南森康三峰生物工程有限公司,山东济南250014 [5]青海亚特生物科技有限公司,青海西宁810000

出　　处：《化学与生物工程》2018年第4期65-68,共4页Chemistry & Bioengineering

基　　金：青海省科技合作专项(2016-HZ-817);山东省三院联合基金项目(ZR2016YL006)

摘　　要：采用反相C18柱色谱、硅胶柱色谱、制备型高效液相色谱成功地从忍冬根的正丁醇萃取物中分离得到6个高纯度单体,通过核磁共振波谱和质谱鉴定其为:绿原酸(Ⅰ)、獐芽菜苷(Ⅱ)、马钱苷(Ⅲ)、断氧化马钱子苷(Ⅳ)、4-O-(4′-O-咖啡酰葡萄糖基)-5-O-咖啡酰奎宁酸(Ⅴ)、3,5-O-二咖啡酰奎宁酸(Ⅵ)。其中,马钱苷(Ⅲ)为首次从忍冬根中分离得到,4-O-(4′-O-咖啡酰葡萄糖基)-5-O-咖啡酰奎宁酸(Ⅴ)为首次从该种属植物中分离得到。该研究为进一步开发利用忍冬植物资源,深入了解其药用活性成分奠定了基础。We separated six high purity monomers from n-butanol extract of Lonicera japonica Thunb.roots by reversed phase C 18 column chromatography,silica gel column chromatography,and preparative high performance liquid chromatography.Moreover,we characterized their chemical structures by nuclear magnetic resonance(NMR)and electrospray ionization mass spectrometry,and identified as chlorogenic acid(Ⅰ),sweroside(Ⅱ),loganin(Ⅲ),secoxyloganin(Ⅳ),4-O-(4′-O-caffeoyl glucosyl)-5-O-caffeoylquinic acid(Ⅴ),and 3,5-O-dicaffeoylquinic acid(Ⅵ),respectively.In which,loganin(Ⅲ)is firstly isolated from Lonicera japonica Thunb.roots,and 4-O-(4′-O-caffeoyl glucosyl)-5-O-caffeoylquinic acid(Ⅴ)is firstly isolated from Lonicera plants.The study lays the foundation of further exploitation and utilization of Lonicera plants and further understanding of medicinal active ingredients.

关 键 词：忍冬 正丁醇 化学成分 绿原酸 马钱苷 

分 类 号：R284.1[医药卫生—中药学]