蚕蛹蛋白粘胶纤维中蛋白质含量的测定  

Determination of protein content in silkworm pupa protein viscose fiber

作　　者：徐杰 李伟坤 李振球 陈颂伟 丁敏 杜文琴[2] XU Jie;LI Weikun;LI Zhenqiu;CHEN Songwei;DING Min;DU Wenqin(Guangdong Jiangmen Supervision Testing Institute of Quality&Metrology,Jiangmen 529000,China;Department of Textile,Wuyi University,Jiangmen 529000,China)

机构地区：[1]广东省江门市质量计量监督检测所,广东江门529000 [2]五邑大学纺织服装学院,广东江门529000

出　　处：《丝绸》2018年第5期8-11,共4页Journal of Silk

基　　金：江门市基础与理论科学研究类科技计划项目(20150030004438)

摘　　要：文章使用全自动凯氏定氮仪测定蚕蛹蛋白粘胶纤维中蛋白质含量,选择样品干重、硫酸铜用量、硫酸钾用量、浓硫酸用量、消化温度和消化时间为试验因素,蛋白质含量为试验指标,利用SPSS软件建立正交试验,通过正交试验设计优化及对试验结果的直观分析和方差分析,得到一套测定蚕蛹蛋白粘胶纤维中蛋白质含量的最优方案,并对最优方案进行验证。最后确定最优方案为:样品干重0.2 g,硫酸铜用量0.2 g,硫酸钾用量10 g,浓硫酸用量12 m L,消化温度420℃,消化时间45 min。The protein content of silkworm pupa protein viscose fiber was determined by automatic Kjeldahl apparatus.Dry weight of sample,copper sulfate content,potassium sulfate dosage,concentrated sulfuric acid dosage,digestion temperature and digestion time were chosen as test factors,and protein content was selected as the test index.SPSS software was used to establish the orthogonal test.Through design optimization of orthogonal test,intuitive analysis and variance analysis of test results,a set of optimal scheme to determine protein content of silkworm pupa protein viscose fiber,and the optimal scheme was verified.The optimal scheme is as follows:dry weight of sample 0.2 g,copper sulfate dosage 0.2 g,potassium sulfate dosage 10 g,concentrated sulfuric acid dosage 12 mL,digestion temperature 420℃and digestion time 45 min.

关 键 词：纺织材料 再生蛋白质纤维 蚕蛹蛋白粘胶纤维 蛋白质含量 凯氏定氮法 正交试验 

分 类 号：TS102.5[轻工技术与工程—纺织工程]