多肽体内外肺癌靶向性鉴定研究  

Study on targeted identification of peptide to lung cancer in vivo and in vitro

作　　者：张正兵 臧林泉[2] ZHANG Zheng-bing;ZANG Lin-quan(Zhang Zhou Health Vocational College,Fujian Zhangzhou 363000,China;Guang Dong Pharmaceutical University,Guangdong Guangzhou 510006,China)

机构地区：[1]漳州卫生职业学院,福建漳州363000 [2]广东药科大学,广东广州510006

出　　处：《临床医药文献电子杂志》2017年第77期15068-15069,共2页Electronic Journal of Clinical Medical Literature

基　　金：国家高科技发展863计划(NO.2012AA020304)

摘　　要：目的鉴定多肽对肺癌的靶向性结合作用。方法采用体外临床肺癌标本组织芯片和体内荷瘤裸鼠组织冰冻切片鉴定的方法,对多条经异硫氰酸荧光素(Fluorescein isothiocyanate isomer I,FITC)标记的多肽进行肺癌结合靶向性、特异性鉴定和阳性结合率统计。结果荷瘤裸鼠体内冰冻切片和体外临床肺癌标本组织芯片鉴定结果显示,3号肽和7号肽,即ZP3和ZP7在肺癌组织具有较强的荧光分布,组织芯片鉴定结果显示,ZP3和ZP7具有对各型肺癌有较高的阳性结合率分别为32.1%和28.4%。结论多肽ZP3和ZP7具有较强的肺癌靶向性,未来对肺癌靶向性药物的开发具有重要的意义。Objective Identification of targeted binding of peptides to lung cancer.Methods Lung Targeting and Specificity of polypeptides signed FITC(Fluorescein isothiocyanate isomer I),will be identified by cancer Tissue microarray vitro and Frozen section of nude mice cancer tissue in vivo.And then Complete statistics positive binding rate.Results Result of cancer Tissue microarray in vitro showed ZP3 and ZP7 had stronger Fluorescence distribution,and result of Tissue microarray showed ZP3 and ZP7 had higher positive binding rate,32.1%and 28.4%.Conclusion Peptides of ZP3 and ZP7 had stronger targeting binging to lung cancer issues,and they had important significance to the new anti-cancer drugs development.

关 键 词：多肽 靶向性 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]