菌株CICC 33077的鉴定及培养基组分的响应面优化  

Identification of strain CICC 33077 and the optimization of medium composition by response surface methodology

作　　者：孙思佳 翟磊[1] 白飞荣 于学健 冯慧军[1] 姚粟[1] SUN Sijia;ZHAI Lei;BAI Feirong;YU Xuejian;FENG Huijun;YAO Su(China National Research Institute of Food and Fermentation Industries,China Center of Industrial Culture Collection,Beijing 100015,China)

机构地区：[1]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100015

出　　处：《中国酿造》2018年第4期44-50,共7页China Brewing

基　　金：国家重点研发计划资助(2016YFD0400502)

摘　　要：以扳倒井芝麻香型白酒高温大曲中分离的菌株CICC 33077为研究对象,采用多相分类学技术确定该菌株的分类学地位;以生物量(OD_(600nm))为响应值,并通过Plackett-Burman试验、最陡爬坡试验以及Box-Behnken试验对其培养基组分进行优化。研究结果表明,菌株CICC 33077鉴定为扣囊复膜孢酵母(Saccharomycopsis fibuligera),最优培养基组分为:麦芽浸粉3.586%,大豆蛋白胨1.216%,蛋白胨1.470%,磷酸氢二钾1.148%;最适生长温度为30℃,最适生长pH值为6.0。菌株CICC 33077优化后的培养基中生长的生物量(OD_(600nm)值)能够达到(8.136±0.102),比优化前提高了170%。Strain CICC 33077 isolated from high-temperature Daqu of Bandaojing sesame-flavor Baijiu was identified by polyphasic taxonomy technique and the compositions of the medium were optimized using Plackett-Burman experiments,the steepest the steepest ascent experiments and Box-Behnken experiments with biomass(OD600 nm)as the response value.The results indicated that strain CICC 33077 was identified as Saccharomy-copsis fibuligera.The optimimum compositions of medium were as follows:malt extract powder 3.586%,soybean peptone 1.216%,peptone 1.470%,dipotassium hydrogen phosphate 1.148%.Meanwhile,the optimum growth temperature and pH value of strain CICC 33077 were 30℃and 6.0,respectively.The biomass(OD600600 nm)reached 8.136±0.102 when strain CICC 33077 was cultured in the optimum medium,which was 170%higher than that of before optimization.

关 键 词：菌种鉴定 扣囊复膜孢酵母 培养基组分 响应面优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]