香菇多糖对口腔鳞癌细胞增殖的影响  

Effect of lentinan on the proliferation of oral squamous cell carcinoma

作　　者：颜亚楠 王晶晶[1] 袁毅 万林忠[2] YAN Yanan;WANG Jingjing;YUAN Yi;WAN Linzhong(Jiangsu Key Laboratory of Oral Diseases,Nanjing Medical University,Nanjing 210029,China;Department of Oral and Maxillofacial Surgery,Affiliated Hospital of Stomatology,Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [2]南京医科大学附属口腔医院口腔颌面外科,江苏南京210029

出　　处：《口腔生物医学》2018年第2期79-81,105,共4页Oral Biomedicine

基　　金：江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的:探讨香菇多糖对口腔鳞癌细胞HN4增殖的影响。方法:体外MTT法检测香菇多糖对HN4细胞的生长抑制作用;AO/EB法研究香菇多糖诱导HN4细胞凋亡的作用;Western blot检测HN4肿瘤细胞中Bcl-2、Bax的表达情况。结果:香菇多糖体外能显著抑制HN4细胞的生长增殖。AO/EB染色表明香菇多糖刺激细胞后,荧光显微镜下能观察到HN4细胞凋亡,出现染色增强,核固缩,染成橙红色。Western blot结果显示香菇多糖能降低HN4细胞中Bcl-2的表达,增加Bax的表达,并增加Bax/Bcl-2的比值。结论:香菇多糖能够体外抑制HN4细胞的生长增殖,并且能够诱导HN4细胞凋亡。Objective:To investigate the effect of lentinan on the proliferation of oral squamous cell carcinoma cell HN4 and its mechanism.Methods:In vitro MTT assay was used to detect the growth inhibition of HN4 cells.The effect of lentinan on the apoptosis of HN4 cells was detected by AO/EB method.The expression of Bcl-2 and Bax in HN4 tumor cells was detected by Western blot.Results:Lentinan could significantly inhibit the growth and proliferation of oral squamous cell carcinoma HN4 cells.AO/EB staining showed that after lentinan stimulated the cells,apoptosis of HN4 cells was observed under a fluorescence microscope,staining increased,nuclear pyknosis and orange-red staining were observed.Western blot results showed that lentinan could decrease the expression of Bcl-2,increase the expression of Bax and increase the ratio of Bax/Bcl-2 in HN4 cells.Conclusions:Lentinan could inhibit the proliferation of oral squamous cell carcinoma HN4 cells in vitro and induce the apoptosis of HN4 cells.

关 键 词：香菇多糖 口腔鳞癌 HN4 抗肿瘤 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]