H3K27三甲基化蛋白可作为MPNST的重要诊断标记物  

H3K27me3 is a highly sensitive diagnostic marker for MPNST

作　　者：宋紫暄 李光明[1] 张静[1] 张卫民[2] 杨吉龙 朱泽[1] SONG Zi-xuan;LI Guang-ming;ZHANG Jing;ZHANG Wei-min;YANG Ji-long;ZHU Ze(Department of Pathogen Biology,Tianjin Medical University,Tianjin 300070,China;Department of Blood Transfusion,The Third Central Hospital of Tianjin,Tianjin 300170,China;Department of Bone and Soft Tissue Tumor,Cancer Institute and Hospital,Tianjin Medical University,National Clinical Research Center for Cancer,Tianjin 300060,China)

机构地区：[1]天津医科大学病原生物学系,天津300070 [2]天津市第三中心医院输血科,天津300170 [3]天津医科大学肿瘤医院骨与软组织肿瘤科,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,天津市恶性肿瘤临床医学研究中心,天津300060

出　　处：《天津医科大学学报》2018年第4期353-356,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金资助项目(81672650)

摘　　要：目的:探讨组蛋白3赖氨酸27位点的三甲基化(H3K27me3)在恶性周围神经鞘瘤(MPNST)中的表达及对预后的影响。方法:收集恶性周围神经鞘瘤病理组织标本43例,经福尔马林固定,石蜡包埋后,利用组织芯片技术(TMA)制成组织芯片,免疫组织化学方法检测H3K27me3等蛋白的表达,根据临床数据和随访信息,运用SPSS 19.0统计软件进行分析比较不同H3K27me3表达水平与患者临床病理特征之间的关系。结果:MPNST组织芯片位点完整。H3K27me3表达缺失组约占65.11%,其中完全缺失组占39.53%,部分缺失组约占25.58%,即以H3K27me3表达缺失为诊断依据则MPNST的检出率可达65.11%,且与原诊断一致;在39例散发病例样本中有43.58%完全缺失、28.20%部分缺失,4例NF1相关样本100%保留表达,即在非NF1相关的MPNST中检出率可达71.78%。结论:检测H3K27me3的表达水平对MPNST的诊断具有良好的灵敏性和特异性,特别是在除外NF1相关临床背景的MPNST前期诊断中具有重要意义,有望成为早期诊断散发型MPNST的生物学标记物。Objective:To investigate the expression and prognostic effect of H3K27 trimethylation protein(H3K27me3)in malignant peripheral nerve sheath tumor(MPNST).Methods:A total of 43 MPNST patients were enrolled into this study.Tissue microarray(TMA)technique and immunohistochemistry(IHC)method were used for H3K27me3 immunostaining.Clinicopathologic and suvival data were carefully collected.The relationship of H3K27me3 expression levels with clinical feature and prognosis of patients were compared by SPSS 19.0.Results:The quality of TMA was perfect and met the standard of analysis.Among all MPNST patients,65.11%were characterized with loss expression of H3K27me3,39.53%were with complete loss expression and 25.58%were with partial loss expression of H3K27me3.If loss expression of H3K27me3 was a diagnostic basis for MPNST,the detectable rate was 65.11%,in accordance with original diagnosis.Among the 39 sporadic MPNST patients,43.58%were with complete loss expression of H3K27me3,and the partial loss patients were 28.20%.All of the type 1 neurofibromatosis(NF1)MPNSTs expressed H3K27me3.The detectable rate was 71.78%outside of NF1 clinical history MPNST.Conclusion:Immunohistochemical analysis of H3K27me3 has good sensitivity and spcificity for the diagnosis of MPNST,particularly outside NF1 clinical history.Loss of H3K27me3 expression may be a sensitive maker for sporadic MPNST.

关 键 词：恶性周围神经鞘瘤 H3K27me3 NF1 组织芯片 

分 类 号：R73[医药卫生—肿瘤]