红景天内生放线菌Hj3发酵条件及其发酵产物抑菌活性初探  

Study on the Fermentation Conditions and Fermentation Products Activity of Endophytic Actinomycetes Hj3 from Rhodiola rosea L.

作　　者：史晓晶 于春艳 韩佳音 王华杰 SHI Xiaojing;YU Chunyan;HAN Jiayin;WANG Huajie(Department of Biology,Xinzhou Teachers University,Xinzhou 034000,China;Xinzhou Food and Drug Administration,Xinzhou 034000,China)

机构地区：[1]忻州师范学院生物系,山西忻州034000 [2]忻州市食品药品监督管理局,山西忻州034000

出　　处：《云南农业大学学报（自然科学版）》2018年第3期422-427,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：忻州师范学院博士科研启动项目;忻州师范学院科研基金资助项目(201716);忻州师范学院大学生科技创新项目(K2017-12-2-8)

摘　　要：【目的】探究红景天内生放线菌——变异链霉菌Hj3(Streptomyces variabilis)的发酵条件、抑菌机制及对采后番茄早疫病的防治效果,为该潜在生防菌的开发利用奠定重要的理论与实践基础。【方法】采用菌丝生长速率法筛选Hj3抑菌物质产生的最佳培养液、接种量,利用正交试验设计探讨培养温度、培养转速、培养液初始p H值等条件,测定Hj3的生长曲线、抑菌物质产生曲线、Hj3抑菌物质对番茄早疫病菌产孢和孢子萌发的影响及对采后番茄早疫病的防治效果。【结果】在4号培养液(乳糖15 g、玉米粉4 g、K_2HPO_4 0.8 g、KCl 0.2 g、CaCO_4 1.6 g、水1 000 m L、p H 7)中接入8%Hj3种子液,28℃150 r/min震荡培养,在第4天时培养液中的活菌数最大,在第5天时Hj3发酵液的抑菌率达到最高;Hj3抑菌物质可明显抑制番茄早疫病菌孢子的产生和萌发,对采后番茄早疫病的防治也有一定效果。【结论】探讨出的发酵条件有利于Hj3抑菌物质的后续研究及应用,进一步明确其抑菌机制为Hj3的实际应用提供了更加充分的理论支持。[Purpose]In order to explore the new potential biocontrol member Hj3(Streptomyces variabilis),the fermentation conditions,inhibition mechanism and control efficiency of postharvest tomato early blight of antifungal substance produced by endophytic actinomycetes Hj3 from Rhodiola rosea were studied.[Method]Using mycelial growth rate method,the best medium and inoculation dose were screened.The cultivated temperature,rotated speed,the initial pH value were discussed by the orthogonal experimental design.And the growth curve of Hj3,antifungal substance produced curve of Hj3,spore production,spore germination of Alternaria solani affected by fermentation broth of Hj3,and the control efficacy of postharvest tomato early blight were tested.[Results]The 8%seed medium of Hj3 was inoculated in the medium with lactose 15 g,corn meal 4 g,K2HPO4 0.8 g,KCl 0.2 g,CaCO4 1.6 g,water 1 000 mL,pH 7.It was incubated in 28℃and shaken with 150 r/min.In the fourth day the viable count was up to maximum,and the inhibition rate of Hj3 fermentation broth was the highest in the fifth day.The antifungal substance of Hj3 could inhibit the spore production and spore germination of A.solani obviously,and control the postharvest tomato early blight well.[Conclusion]The fermented condition avails the further study and application of antifungal substance produced by Hj3.And the inhibition mechanism and control efficacy of postharvest tomato early blight could support theoretically for practical application of Hj3.

关 键 词：红景天内生放线菌 番茄早疫病菌 发酵条件 抑菌活性 

分 类 号：S476.91[农业科学—农业昆虫与害虫防治]