大曲曲药中脲酶功能菌的选育与应用  

Breeding and Application of a Urease-Producing Strain from Daqu

作　　者：徐占成[1] 徐姿静[1] 唐清兰[1] 刘孟华[1] XU Zhancheng;XU Zijing;TANG Qinglan;LIU Menghua(Jiannanchun Group Co.Ltd.,Mianzhu,Sichuan 618200,China)

机构地区：[1]四川剑南春集团有限责任公司,四川绵竹618200

出　　处：《酿酒科技》2018年第7期98-103,共6页Liquor-Making Science & Technology

摘　　要：以剑南春中温大曲为材料,通过平板和产酶能力的筛选,分离得到1株产脲酶性能稳定的JNC-U5菌株,该菌经形态、生理生化及18S r DNA测序鉴定定性为聚多曲霉。该菌产脲酶活力可达到69.3 U/g,其酶活在pH3.5~4.5环境下仍保持较高活性,应用于酿酒生产可大幅降低EC含量,确保白酒的食品安全性。A strain with stable urease catalytic activity named as JNC-U5,was isolated from Jiannanchun medium-temperature Daqu by traditional plate method and urease-producing capacity screening.Based on polyphasic study,phenotypic characteristics,physiological-biochemical properties and 18S rDNA sequence analysis,JNC-U5 was identified as Aspergillus sydowii.Its urease-producing capacity could reach 69.3 U/g,and its enzyme activity remained high at pH 3.5 to 4.5.Its use in liquor-making could greatly reduce EC content and ensure liquor safety.

关 键 词：功能菌 脲酶 聚多曲霉 剑南春中温大曲 18SrDNA 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]