生长猪不同蛋白源日粮必需氨基酸释放规律探讨  

Study on the Release Kinetics of Amino Acids of Different Protein Sources in Growing Pigs Diets

作　　者：王进 孙泽威[1,2] WANG JIN;SUNZe-wei(College of Animal Science and Technology,Jilin Agricultural University,Ji lin Changchun 130118,China;Jilin Province Key Laboratory of Animal Nutrition and Feed Science,Jilin Agricultural University,Jilin Changchun 130118,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林农业大学动物营养与饲料学科重点实验室,吉林长春130118

出　　处：《中国畜牧杂志》2018年第7期59-65,共7页Chinese Journal of Animal Science

基　　金：国家重点基础研究发展计划(973计划)(2013CB127306)

摘　　要：本研究旨在探究不同蛋白源日粮的氨基酸释放规律,为进一步实现日粮氨基酸供给与动物需求的动态平衡提供理论依据。试验以豆粕玉米蛋白粉、鱼粉、发酵豆粕构建不同蛋白源组成日粮,模拟猪胃肠环境,对4组日粮进行体外消化试验,对不同蛋白源日粮的氨基酸释放进行分析。结果表明:4组日粮中总游离氨基酸含量存在显著差异(P<0.05),氨基酸水平由高到低依次表现为豆粕组、玉米蛋白粉组、鱼粉组、酵豆粕组;体外消化4~8 h,玉米蛋白粉组和发酵豆粕组的赖氨酸释放量显著高于其他2组(P<0.05);豆粕组蛋氨酸释放量最高;鱼粉组和发酵豆粕组的苯丙氨酸释放速度显著低于豆粕组和玉米蛋白粉组(P<0.05),但4组日粮间苏氨酸的释放未表现出显著差异;体外消化12~20 h,发酵豆粕组4种必需氨基酸的释放量始终高于其他3组,玉米蛋白粉组日粮的4种必需氨基酸释放量均处于最低水平;体外消化24~28 h,4组日粮间赖氨酸、蛋氨酸、苏氨酸、苯丙氨酸的累积释放量差异不显著。在等能、等氮、氨基酸平衡条件下,通过调整日粮的蛋白源结构,可以有效地调控日粮在动物消化道内的氨基酸释放模式,进而促进日粮氨基酸供给与动物需求的动态平衡,以提高动物的日粮养分利用效率、生产性能,并促进动物的生理健康。The aim of this study was to explore the kinetics of amino acid release from different protein diet sources and to provide a theoretical basis for further realization of dietary amino acid supply and dynamic balance to meet animal needs.In this study,The experiment consisted of constructing different protein sources in soybean meal,corn meal,fish meal and fermented soybean meal to form a diet.In order to simulate the gastrointestinal environment of pig.In vitro digestibility experiment where amino acid concentrations in digestive juice after in vitro digestion were measured by ultra-high performance liquid chromatography(UPLC),and amino acid release profiles of diets containing different protein sources were analyzed.The results showed that Due to the different amount of synthetic amino acids,there were significant differences in total free amino acids among the four groups.After 4 to 8 hours of in vitro digestion,the Lys release of corn gluten meal and fermented soybean meal was significantly higher than that of the other two groups.Met release was highest in the soybean meal group,and the release rate of Phe was significantly lower in the fish meal and fermented soybean meal groups than in the soybean meal group.In the corn gluten meal group,there was no significant difference in the release of Thr among the four diets.After in vitro digestion for 12 h to 20 h,the release of the four essential amino acids in the fermented soybean meal group was always higher than those in the other three groups,and the release of the four essential amino acids in the diet of the corn gluten meal group was at the lowest level.There was no significant difference between the soybean meal group and the fish meal group.In vitro digestion to 24~28 h,the cumulative release of Lys,Met,Thr,Phe among the four groups of diets was not significant.The following conclusions can be drawn that the amino acid release pattern of diets in the digestive tract of animals can be effectively controlled by adjusting the protein source structure of the

关 键 词：蛋白源 游离氨基酸 释放动态 猪 

分 类 号：S828.5[农业科学—畜牧学]