响应面法优化重组大肠杆菌发酵生产藻蓝蛋白培养基的关键介质组分  

Optimization of Critical Medium Components for Phycocyanin Production by Recombinant Escherichia coli Using Response Surface Methodology

作　　者：于平[1] 徐超超 朱鹏志 YU Ping;XU Chaochao;ZHU Pengzhi(School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310035,China)

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310035

出　　处：《食品科学》2018年第16期161-167,共7页Food Science

基　　金：国家自然科学基金面上项目(31171658);浙江省教育厅重点项目(Z201010029);食品科学与工程浙江省重中之重一级学科项目(2017SIAR214)

摘　　要：探讨响应面法优化重组大肠杆菌发酵生产藻蓝蛋白培养基的关键介质组分,确定其最佳用量。首先通过部分因素试验设计评价影响重组大肠杆菌发酵生产藻蓝蛋白产量的相关因素,筛选出具有显著效应的关键因素,然后采用响应面法确定关键因素的最佳质量浓度。筛选出的3个具有显著效应的关键因素为蛋白胨用量、酵母提取物用量和K_2HPO_4·3H_2O用量,其最佳用量分别为23.8、13.4 g/L和9.4 g/L,在该条件下获得的藻蓝蛋白质量浓度为15.28 mg/L。本研究结果为藻蓝蛋白的产业化生产和应用提供了一定实验支撑。The critical medium components for phycocyanin production by recombinant Escherichia coli were selected and optimized using response surface methodology.The medium components with significant influence on phycocyanin yield were identified using a fractional factorial design and their levels were optimized using response surface methodology.Peptone,yeast extract and K2HPO4·3H2O were recognized as the critical medium components,and their optimal levels were determined as 23.8,13.4 and 9.4 g/L,respectively,which gave a phycocyanin yield of 15.28 mg/L.This study can pave a good foundation for future production and use of phycocyanin.

关 键 词：重组大肠杆菌 藻蓝蛋白 发酵 响应面法 优化 

分 类 号：Q815[生物学—生物工程]