蜂胶提取物对顺铂诱导大鼠肝、肾损伤的保护作用  

Protective Effect of Propolis Extract on Liver and Kidney Injury Induced by Cisplatin in Rats

作　　者：黄海波 沈圳煌 耿倩倩 史培颖[1,3,4] 吴珍红 孙燕如[1,3] 韩鸣凤 缪晓青 HUANG Haibo;SHEN Zhenhuang;GENG Qianqian;SHI Peiying;WU Zhenhong;SUN Yanru;HAN Mingfeng;MIAO Xiaoqing(Apitherapy Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;State and Local Joint Engineering Laboratory of Natural Biotoxin,Fuzhou 350002,China;College of Bee Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学蜂疗研究所,福建福州350002 [2]福建农林大学食品科学学院,福建福州350002 [3]天然生物毒素国家地方联合工程实验室,福建福州350002 [4]福建农林大学蜂学学院,福建福州350002

出　　处：《食品科学》2018年第15期159-164,共6页Food Science

基　　金：农业部蜂产业体系蜂产品保健功能岗位科学家项目(CARS-45-KXJ19)

摘　　要：目的:探究蜂胶乙醇提取物对顺铂所致肝、肾损伤的干预作用。方法:采用不同剂量(50、100、150 mg/kg mb)的蜂胶乙醇提取物对大鼠进行灌胃,连续7 d给药后单次腹腔注射10 mg/kg mb的顺铂建立肝、肾损伤模型,之后再灌胃5 d。实验结束后通过测定血清中肝、肾损伤的标志物,肝、肾组织中脂质过氧化物、抗氧化物的含量以及观察肝、肾组织病理学的改变来探究蜂胶对顺铂毒性的干预作用。结果:与模型组相比,高剂量组大鼠血清中谷草转氨酶、谷丙转氨酶的活力,尿素氮、肌酐含量均有降低;肝、肾组织中超氧化物歧化酶、过氧化氢酶的活力,还原型谷胱甘肽的含量均有升高;丙二醛、诱导型一氧化氮合酶含量均有降低;从组织病理学方面来看,模型组的肝、肾组织都出现了病变,表现为肝小叶细胞坏死,肾小球细胞脱落、空泡性等,蜂胶给药组这些病变情况均有不同程度的降低。结论:蜂胶乙醇提取物可以降低体内的氧化应激程度,提高机体自身抗氧化能力,从而很好地降低顺铂造成的肝、肾损伤。Objective:To investigate the intervention effect of ethanolic extract of propolis(EEP)on liver and kidney injury induced by cisplatin(CP).Methods:Rats were treated by intragastric administration with different doses of EEP(50,100 and 150 mg/kg mb)for 7 days,and then the liver and kidney injury models were established by a single intraperitoneal injection of CP(10 mg/kg mb)at 2 h after the last administration.Finally,the rats were intragastrically administrated for another 5 days.The intervention effect of EEP on cisplatin toxicity was evaluated by measuring the markers of liver and kidney injury in serum and the levels of lipid peroxidation and antioxidants in liver and kidney and observing the pathological changes of liver and kidney.Results:EEP significantly reduced cisplatin-induced toxicity in rat liver and kidney.Compared with the model group,the activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT),and the contents of blood urea nitrogen(BUN)and creatinine(Cr)in serum were decreased in the high-dose group.The activities of superoxide dismutase(SOD)and catalase(CAT),and the content of reduced glutathione(GSH)in liver and kidney were increased.The contents of malondialdehyde(MDA)and inducible nitric oxide synthase(iNOS)were decreased.The histopathologic examination indicated that the pathological changes of liver and kidney were observed in the model group,with hepatic lobule necrosis and the abscission and cavitation of glomerular cells.These pathological changes were attenuated to varying degrees in the EEP treatment groups.Conclusion:Ethanolic extract of propolis can reduce cisplatin-induced liver and kidney damage by reducing the level of oxidative stress and improving the antioxidant capacity of the body.

关 键 词：蜂胶 顺铂毒性 肝损伤 肾损伤 氧化应激 

分 类 号：TS201.4[轻工技术与工程—食品科学]