采后O_3处理对采前CPPU处理猕猴桃果实乙烯代谢的影响  

Effect of Postharvest O_3 Treatment on Ethylene Metabolism of Kiwifruit Preharvest Treated with CPPU

作　　者：苏苗[1] 罗安伟[1] 李琳[1] 李圆圆[1] 白俊青 李锐 方沂蒙 宋俊奇 蔺志颖 刘占德[2] SU Miao;LUO AnWei;LI Lin;LI YuanYuan;BAI JunQing;LI Rui;FANG YiMeng;SONG JunQi;LIN ZhiYing;LIU ZhanDe(College of Food Science and Engineering Northwest A&F University,Yangling 712100,Shaanxi;College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《中国农业科学》2018年第15期2980-2989,共10页Scientia Agricultura Sinica

基　　金：陕西省农业科技创新与攻关项目(2015NY051);陕西省重点研发计划农业领域重点项目(2018ZDXM-NY-056);杨凌示范区产学研用协同创新重大项目(2018CXY-04)

摘　　要：【目的】探究臭氧(ozone,O_3)是否能有效减轻膨大剂(N-2-氯-4-吡啶基苯-N’-苯基脲,CPPU)对猕猴桃带来的负面影响,为市场上使用CPPU猕猴桃的贮藏提供信息。【方法】以采前盛花期28d使用20 mg·L^(-1) CPPU处理和对照用清水蘸果处理的秦美猕猴桃为试验材料,研究不同浓度的O_3处理(0、10、40、70 mg·m^(-3))对贮藏期间猕猴桃果实乙烯代谢过程中的蛋氨酸(methionine,Met)、S-腺苷蛋氨酸(S-adenosyl methionine,SAM)、1-氨基环丙烷-1-羧酸(1-aminocyclopropane-1-carboxylic acid,ACC)含量及其相关代谢酶1-氨基环丙烷-1-羧酸合成酶(ACC synthase,ACS)和1-氨基环丙烷-1-羧酸氧化酶(ACC oxidase,ACO)活性的影响。【结果】CK组(未使用CPPU也未使用O_3处理的为CPPU对照组CK)在贮藏过程中Met、SAM含量以及SAM合成酶、ACS、ACO活性的下降速率均低于CK1组(使用CPPU但未使用O_3处理的为臭氧处理对照组CK1);贮藏60 d时,CK1和各O_3处理(10、40、70 mg·m^(-3))的Met含量分别为1.36、2.62、4.41和2.60 mg·(100 g)^(-1),O_3处理显著高于CK1(P<0.05);CK1和40 mg·m^(-3) O_3处理的SAM含量分别为15.48 mg·(100 g)^(-1)和20.73 mg·(100 g)^(-1),具有显著性差异(P<0.05),而CK1和10 mg·m^(-3) O_3处理组、70 mg·m^(-3) O_3处理组无显著性差异(P>0.05);CK1和各O_3处理(10、40、70 mg·m^(-3))的ACC含量分别为0.068、0.059、0.038和0.055 nmol·g^(-1),40、70 mg·m^(-3 )O_3处理与CK1具有显著性差异(P<0.05);CK1和各O_3处理(10、40、70 mg·m^(-3))的ACS活性分别为0.084、0.069、0.054和0.080 nmol·(g·h)^(-1);ACO活性的峰值分别为0.062、0.046、0.029和0.051 nmol·(g·h)^(-1),O_3处理和CK1之间存在显著性差异(P<0.05);CK1和各O_3处理(10、40、70 mg·m^(-3))的乙烯的峰值分别为18.42、15.99、9.86、11.69μL·kg^(-1)·h^(-1);呼吸高峰分别是18.77、16.15、12.24、15.48 mg·kg^(-1)·h^(-1)。【结论】CPPU增加了猕猴桃乙烯释放量,加速了果实软化,对猕猴桃贮藏带来负面【Objective】The objective of this study was to investigate whether or not the ozone(ozone,O3)could effectively reduce the negative effects of the swelling agent(N-2-chloro-4-pyridine benzene-N'-phenyl urea,CPPU)on kiwifruit,which would provide useful information for using of CPPU on the market for the storage.【Method】First,the Qinmei kiwifruit was treated with 20 mg·L^-1 CPPU in the growing season,then treated with 10,40,70 mg·m^-3 ozone respectively in postharvest.The material contents in kiwifruit of methionine(methionine,Met),S-adenosyl methionine(S-adenosyl methionine,SAM),1-aminocyclopropane-1-carboxylic acid(1-aminocyclopropane-1-carboxylic acid,ACC)and the related metabolic enzyme activity of ACC synthase(ACC synthase,ACS)and ACC oxidase(ACC oxidase,ACO)were studied.【Result】The decreasing rate of Met,SAM and activity of SAM synthase,ACS,ACO under CK(without the use of CPPU and O3 treatment as CPPU control CK group)during storage was lower than that under CK1(using CPPU without using O3 treatment for ozone treatment control CK1 group).At 60 days after kiwifruit storage,the contents of Met under CK1 and each treatment group(10,40 and 70 mg·m^-3 ozone)were 1.36,2.62,4.41 and 2.60 mg·(100 g)-1,respectively.The contents under O3 treatment was significantly higher than that under CK1(P<0.05).The contents of SAM under CK1 and 40 mg·m^-3 O3 group were 15.48 mg·(100 g)-1 and 20.73 mg·(100 g)-1,respectively(P<0.05),but the contents under CK1 10 mg·m^-3 O3 treatment,and 70 mg·m^-3 O3 treatment no significant difference(P>0.05).The ACC contents under CK1 and each treatment group(10,40 and 70 mg·m^-3 ozone)were 0.068,0.059,0.038 and 0.055 nmol·g-1,respectively.There were significant differences(P<0.05)between 40,70 mg·m^-3 O3 treatments and CK1.The ACS activities in CK1 group and each treatment group(10,40and 70 mg·m^-3)were 0.084,0.069,0.054 and 0.080 nmol·(g·h)^-1,respectively.The peak of ACO activity was 0.062,0.046,0.029 and 0.051 nmol·(g·h)^-1,respectively.There was significant differen

关 键 词：臭氧 膨大剂 猕猴桃 乙烯代谢 呼吸速率 

分 类 号：S663.4[农业科学—果树学]