绍兴黄酒熟麦曲的宏蛋白质组的样品制备方法研究  

Research of Preparation Method for the Metaproteome in Shaoxing Rice Wine Koji

作　　者：张波[1] 陆健[2] Zhang Bo;Lu Jian(Department of Applied Engineering,Zhejiang Economic and Trade Polytechnic,Hangzhou 310018,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]浙江经贸职业技术学院应用工程系,浙江杭州310018 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《现代食品》2018年第14期121-124,共4页Modern Food

基　　金：浙江省教育厅一般科研项目(编号:Y201636636)

摘　　要：本研究以绍兴黄酒熟麦曲为研究对象,采用丙酮法和Clean Up Kit试剂盒法来处理样品,发现Clean Up Kit试剂盒法能够有效去除盐离子、多糖等干扰物质,蛋白点数目较多且清晰,无拖尾现象。蛋白样品上样量为90μg时,分离效果较好。初步建立适用于绍兴黄酒熟麦曲的宏蛋白质组的样品制备方法,获得了较好的双向电泳图谱。In this study,Shaoxing koji was the research object,two different preparation methods of sample,acetone precipitation and Clean Up Kit were used to isolate the metaproteome.The results showed that Clean Up Kit can effectively remove salt ions,polysaccharides and other,the number of protein spots was more and clear.When the amount of protein was 90μg,the metapromics spots were effiectively separated.In conclusion,a suitable preparation method for metaproteome from Shaoxing rice wine koji was established,the two-dimensional electorphoresis maps with good quality were obtained.

关 键 词：绍兴黄酒 熟麦曲 宏蛋白质组学 制备方法 

分 类 号：TS262.4[轻工技术与工程—发酵工程]