苦荞D-手性肌醇提取物抑制NOX4活性及提高血管舒张作用  

D-Chiro-Inositol-Enriched Tartary Buckwheat Bran Extract Inhibits NOX4 Activity and Improves Endothelium-Dependent Relaxation in Vessels

作　　者：张波波 高彩凤[1] 李云龙 王敏[1] ZHANG Bobo;GAO Caifeng;LI Yunlong;WANG Min(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China;Institute of Agricultural Products Processing,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]山西省农业科学院农产品加工研究所,山西太原030031

出　　处：《食品科学》2018年第17期165-170,共6页Food Science

基　　金：现代农业产业技术体系建设专项(CARS-08-D2-01)

摘　　要：探讨苦荞麸皮提取物(tartary buckwheat bran extract,TBBE)D-手性肌醇改善脂毒性造成的内皮功能损伤作用。结合体外及体内实验,采用棕榈酸(palmitic acid,PA)刺激内皮原代细胞及大鼠胸主动脉血管,或高脂饲喂(high fat diet,HFD)小鼠造模,考察TBBE对内皮细胞、离体血管及小鼠血管内膜功能的影响。体外结果显示,提取物在不大于50μg/m L剂量下对内皮细胞无损伤作用;不同剂量TBBE显著抑制PA引起的细胞活性氧(reactive oxygen species,ROS)产生及NADPH氧化酶(NADPH oxidase,NOX)4激活;抑制因PA介导的离体血管NOX4激活,改善血管内皮依赖性舒张。小鼠体内实验结果表明,TBBE有效抑制HFD导致的小鼠血管NOX4激活及降低血清游离脂肪酸含量。但HFD组及TBBE干预均不影响小鼠血糖、附睾脂肪系数、肝脏系数及体质量。说明脂毒性明显造成内皮氧化应激,TBBE通过抑制NOX4/ROS通路保护小鼠内皮功能。The protective effect of D-chiro-inositol-enriched tartary buckwheat bran extract(TBBE)on endothelial dysfunction was investigated in the current study.Endothelial cells and the aorta of rats were treated with palmitic acid(PA)alone or in combination with TBBE,and mice were fed a high fat diet(HFD)and then were either orally administered or not with TBBE.Results showed that TBBE at 0?50μg/mL had no harmful effect on cells.TBBE at different concentrations inhibited reactive oxygen species(ROS)production and NADPH oxidase 4(NOX4)activity in PA-stimulated cells.TBBE also reduced NOX4 over-expression and improved endothelium-dependent relaxation in the aorta of PA-treated rats.In HFD mice,oral administration of TBBE reduced blood free fatty acid,but had no effect on blood glucose,epididymal fat coefficient,liver coefficient or body mass.TBBE also prevented NOX4 activity in the aorta endothelium,confirming the beneficial effect of TBBE on endothelial function in vivo under lipid-challenged conditions.Lipid disorder significantly induced oxidative stress in endothelial cells.However,TBBE ameliorated endothelial dysfunction through NOX4/ROS signal pathway.

关 键 词：苦荞麸皮提取物D-手性肌醇 棕榈酸 高脂 NADPH氧化酶4 内皮功能紊乱 

分 类 号：R122.7[医药卫生—环境卫生学]