核桃分心木化学成分及抗炎活性研究  

Chemical Constituents of Diaphragma juglandis Fructus and Their Anti-inflammatory Activity

作　　者：王丹[1,2] 董红敬[2] 郝翠 张力思 郭兰萍[2] 王岱杰 王晓 WANG Dan;DONG Hong-jing;HAO Cui;ZHANG Li-si;GUO Lan-ping;WANG Dai-jie;WANG Xiao(College of Food Science and Engineering,Shandong Agricultural University,Taian 271018,China;Key Laboratory of TCM Quality Control Technology,Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Shandong Institute of Pomology,Shandong Academy of Agricultural Sciences,Taian 271000,China)

机构地区：[1]山东农业大学食品科学与工程学院,泰安271018 [2]齐鲁工业大学(山东省科学院)山东省分析测试中心山东省中药质量控制技术重点实验室,济南250014 [3]山东省果树研究所山东省农业科学院,泰安271000

出　　处：《天然产物研究与开发》2018年第9期1548-1553,1607,共7页Natural Product Research and Development

基　　金：郭兰萍院创新工程先导专项;郭兰萍泰山学者岗位平台;国家自然科学基金青年基金(21506119)

摘　　要：为研究核桃分心木的化学成分,明确其药效物质基础,采用高速逆流色谱、反相C18柱色谱、制备型高效液相色谱等方法进行分离纯化,通过质谱、核磁共振波谱等多种谱学数据分析鉴定其化学结构。从核桃分心木的乙酸乙酯萃取部位分离纯化得到10个化合物,分别鉴定为:2,3-dihydro-3-hydroxy-2-oxo-1H-indole-3-acetic acid(1)、核桃素D(2)、2-hydroxycinchoninic acid(3)、C-藜芦酰乙二醇(4)、槲皮素-3-O-(6″-没食子酰基)-β-D-半乳糖苷(5)、槲皮素-3-O-(6″-没食子酰基)-β-D-葡萄糖苷(6)、金丝桃苷(7)、异槲皮苷(8)、4,8-二羟基-1-四氢萘醌(9)、槲皮素(10)。化合物3,7和8是首次从核桃分心木中分离得到,化合物1、4和6为首次从该种属植物中分离得到。运用Griess法检测LPS诱导的RAW264. 7细胞中一氧化氮(NO)水平,对各单体化合物的抗炎活性进行初步研究,结果显示化合物10具有较好的抗炎作用。To study the chemical constituents of ethyl acetate extract from Diaphragma juglandis Fructus for clarifying its pharmacological basis,the high-speed counter-current chromatography,reversed phase C 18 column chromatography and preparative high performance liquid chromatography were used for the separation and purification.Their chemical structures were identified by spectroscopic datas including electrospray ionization mass spectrometry(ESI-MS)and nuclear magnetic resonance(NMR).Ten compounds were isolated and their chemical structures were identified as 2,3-dihydro-3-hydroxy-2-oxo-1 H-indole-3-acetic acid(1),juglanin D(2),2-hydroxycinchoninic acid(3),C-veratroylglycol(4),quercetin 3-O-(6″-O-galloyl)-β-galactoside(5),quercetin 3-O-(6″-O-galloyl)-β-glucoside(6),hyperoside(7),isoquercitrin(8),isosclerone(9)and quercetin(10).Compound 3,7 and 8 are isolated from Diaphragma juglandis Fructus for the first time and compound 1,4 and 6 are found in this genus for the first time.The anti-inf1ammatory activity of isolated compounds was evaluated by the levels of NO in LPS-induced RAW264.7 tested by Griess reagent.And compound 10 showed strong anti-inf1ammatory activity.

关 键 词：分心木 化学成分 鉴定 抗炎活性 

分 类 号：R285.5[医药卫生—中药学] Q946.9[医药卫生—中医学]